Ulvan is a sulfated heteropolysaccharide present in the cell wall of Ulva species with unique structural properties and technological potential. Here we characterized by FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in nutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger signals of sulfate groups than SEA. 1 H and 13 C NMR showed that both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and type B. SEA ulvan presented signals characteristics of xylose, suggesting the presence of ulvanobiose in its structure, while CULT presented most signals of type A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements of quality and quantity.
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