Biofilms are being engineered in-vitro to produce numerous commodities like biofertilizers, pharmaceuticals, biofuels and electricity, the efficacies of which rely on the biochemicals secreted by the biofilms i.e. extracellular polymeric substances (EPS). It has been shown that once EPS-biochemicals of developed biofilms are applied to an ecosystem, they can restore degraded complex eco system networks for improved ecosystem functioning and sustainability . Identification of the EPS biochemicals and understanding their contributions to the network interactions in particular, are at initial stage. In the present study, using Aspergillus niger , Nostoc sp., and gram (-) Stenotrophomonas maltophilia & gram (+) Bacillus subtilis as test fungal (F), cyanobacterial (C), and bacterial (B) counterparts, respectively we analyzed morphology and bi ochemical parameters of fungal-bacterial (FBBs), fungal-cyanobacteri al (FCBs), cyanobacterial-bacterial (CBBs), and fungal-cyanobacterial-bacterial biofilms (FCBBs). Results revealed that the FCBBs produced the highest concentrat ions of lipids, proteins, and polysaccharides whereas FBBs generated t he highest diversity of biochemicals. Bacterial type ( i.e. gram + or -) and microbial composition in the biofilm affected the biochemical production. Ecologically and industrially important diverse biochemicals which are used individually as medicines, bioremediating agents and industrial chemicals in human society with certain adverse and beneficial effects were detected in the biofilm-EPS. However, in the nature, simultaneous action of those diverse biochemicals applied as biofertilizers has already shown a huge potential to restore the entire agroecosystems degraded due to farmers’ detrimental practices. This striking difference in utilization of the biochemicals and their enhanced effect when they act simultaneously needs further investigations for their better applications.
Favre, L., Ortalo-Magné, A., Pichereaux, C., Gargaros, A., Burlet-Schiltz, O., Cotelle, V. and Culioli, G. (2018) Metabolome and Proteome Changes between Biofilm and Planktonic Phenotypes of the Marine Bacterium Pseudoalteromonas lipolytica TC8. Biofouling, 34, 132-148. https://doi.org/10.1080/08927014.2017.1413551
Resch, A., Rosenstein, R., Nerz, C. and Gotz, F. (2005) Differential Gene Expression Profiling of Staphylococcus aureus Cultivated under Biofilm and Planktonic Conditions. Applied and Environmental Microbiology, 71, 2663-2676.
Flemming, H.C. and Wingender, J. (2010) The Biofilm Matrix. Nature Reviews Microbiology, 8, 623-633. https://doi.org/10.1038/nrmicro2415
Seviour, T., Derlon, N., Dueholm, M.S., Flemming, H.C., Girbal-Neuhauser, E., Horn, H., Kjelleberg, S., van Loosdrecht, M.C., Lotti, T., Malpei, M.F. and Nerenberg, R. (2019) Extracellular Polymeric Substances of Biofilms: Suffering from an Identity Crisis. Water Research, 151, 1-7. https://doi.org/10.1016/j.watres.2018.11.020
Seneviratne, G., Kecskés, M.L. and Kennedy, I.R. (2008) Biofilmed Biofertilisers: Novel Inoculants for Efficient Nutrient Use in Plants. In: Kennedy, I.R., Choudhury, A.T.M.A., Kecskés, M.L. and Rose, M.T., Eds., Efficient Nutrient Use in Rice Production in Vietnam Achieved Using Inoculant Biofertilisers, Australian Centre for International Agricultural Research, Canberra, 126-130. http://biogro.com.vn/wp-content/uploads/ACIAR-PR130-onlineKennedyetal- BioGro-Research.pdf#page=127
Premarathna, M., Seneviratne, G., Ketipearachchi, K.G., Pathirana, A., Karunaratne, R.K.C., Balasooriya, W.K. and Fonseka, K. (2021) Biofilm Biofertilizer Can Reinstate Network Interactions for Improved Rice Production. Ceylon Journal of Science, 50, 235-242.
Seneviratne, G. and Premarathna, M. (2020) Biofilm Medicines: Next-Generation Drugs. https://doi.org/10.13140/RG.2.2.24088.14081
Di Martino, P. (2018) Extracellular Polymeric Substances, a Key Element in Understanding Biofilm Phenotype. AIMS Microbiology, 4, 274-288. https://doi.org/10.3934/microbiol.2018.2.274
Higa, T. and Wididana, G.N. (1991) The Concept and Theories of Effective Microorganisms. Proceedings of the First International Conference on Kyusei Nature Farming, Khon Kaen, 17-21 October 1989, 118-124.
Seneviratne, G. (2003) Development of Eco-Friendly, Beneficial Microbial Biofilms. Current Science, 85, 1395-1396.
Brenner, K., You, L. and Arnold, F.H. (2008) Engineering Microbial Consortia: A New Frontier in Synthetic Biology. Trends in Biotechnology, 26, 483-489. https://doi.org/10.1016/j.tibtech.2008.05.004
Rosche, B., Li, X.Z., Hauer, B., Schmid, A. and Buehler, K. (2009) Microbial Biofilms: A Concept for Industrial Catalysis? Trends in Biotechnology, 27, 636-643. https://doi.org/10.1016/j.tibtech.2009.08.001
Rennie, R.J. (1981) A Single Medium for the Isolation of Acetylene-Reducing (Dinitrogen-Fixing) Bacteria from Soils. Canadian Journal of Microbiology, 27, 8-14. https://doi.org/10.1139/m81-002
Dorina, S., Judith, S., Bjorn, W., Julia, S., Andrea, S., Muffler, K. and Roland, U. (2020) A New Strategy for a Combined Isolation of EPS and Pigments from Cyanobacteria. Journal of Applied Phycology, 32, 1729-1740. https://doi.org/10.1007/s10811-020-02063-x
Ferro, L., Gojkovic, Z., Gorzsás, A. and Funk, C. (2019) Statistical Methods for Rapid Quantification of Proteins, Lipids, and Carbohydrates in Nordic Microalgal Species Using ATR-FTIR Spectroscopy. Molecules, 24, Article No. 3237. https://doi.org/10.3390/molecules24183237
Bandara, W.M.M.S., Seneviratne, G. and Kulasooriya, S.A. (2006) Interactions among Endophytic Bacteria and Fungi: Effects and Potentials. Journal of Bioscience, 31, 645-650. https://doi.org/10.1007/bf02708417
Herath, H.M.L.I., Senanayeke, D.M.N., Seneviratne, G. and Bandara, D.C. (2013) Variation of Biochemical Expressions of Developed Fungal-Bacterial Biofilms over Their Monocultures and Its Effect on Plant Growth. Tropical Agricultural Research, 24, 186-192.
Seneviratne, G. (2019) Microbial Biofilm Engineering: A Next-Generation Biotechnological Application (Keynote Speech). The International Conference on Biotechnology and Food Science (IAECST 2019), Guangzhou, 20-22 December 2019.
Davies, J. (2013) Specialized Microbial Metabolites: Functions and Origins. The Journal of Antibiotics, 66, 361-364. https://doi.org/10.1038/ja.2013.61
Stonik, V. and Stonik, I. (2015) Low-Molecular-Weight Metabolites from Diatoms: Structures, Biological Roles and Biosynthesis. Marine Drugs, 13, 3672-3709. https://doi.org/10.3390/md13063672
Wu, K., Li, W., Song, J. and Li, T. (2015) Production, Purification, and Identification of Cholest-4-en-3-One Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System. Biochemistry Insights, 8S1, BCI-S21580. https://doi.org/10.4137/BCI.S21580
Papoutsis, K., Grasso, S., Menon, A., Brunton, N.P., Lyng, J.G., Jacquier, J.C. and Bhuyan, D.J. (2020) Recovery of Ergosterol and Vitamin D2 from Mushroom Waste-Potential Valorization by Food and Pharmaceutical Industries. Trends in Food Science & Technology, 99, 351-366. https://doi.org/10.1016/j.tifs.2020.03.005
Hammond, B., Katzenellenbogen, B.S., Krauthammer, N. and McConnell, J. (1979) Estrogenic Activity of the Insecticide Chlordecone (Kepone) and Interaction with Uterine Estrogen Receptors. Proceedings of the National Academy of Sciences of the United States of America, 76, 6641-6645.
Kalam, M.A., Alkholief, M., Badran, M., Alshememry, A. and Alshamsan, A. (2020) Co-Encapsulation of Metformin Hydrochloride and Reserpine into Flexible Liposomes: Characterization and Comparison of in Vitro Release Profile. Journal of Drug Delivery Science and Technology, 57, Article ID: 101670. https://doi.org/10.1016/j.jddst.2020.101670
Johra, F.T., Bepari, A.K., Bristy, A.T. and Reza, H.M. (2020) A Mechanistic Review of β-Carotene, Lutein, and Zeaxanthin in Eye Health and Disease. Antioxidants, 9, Article No. 1046. https://doi.org/10.3390/antiox9111046
Choi, S.J., Kim, M.J., Heo, H.J., Kim, J.K., Jun, W.J., Kim, H.K., Kim, E.K., Kim, M.O., Cho, H.Y., Hwang, H.J., Kim, Y.J. and Shin, D.H. (2009) Ameliorative Effect of 1,2-Benzenedicarboxylic Acid Dinonyl Ester against Amyloid Beta Peptide-Induced Neurotoxicity. Amyloid, 16, 15-24. https://doi.org/10.1080/13506120802676997
Chou, K.M., Krapcho, A.P., Horn, D. and Hacker, M. (2002) Characterization of Anthracenediones and Their Photoaffinity Analogs. Biochemical Pharmacology, 63, 1143-1147. https://doi.org/10.1016/S0006-2952(02)00855-9
Chao, X., Zhou, X.J., Dong, C.H. and Zheng, G. (2014) The Effect and Mechanism of Apoptosis on Hela Cells Induced by Bufotalin. Advanced Materials Research, 834-836, 568-572. https://doi.org/10.4028/www.scientific.net/AMR.834-836.568
You, C.L. and Hou, H.M. (2011) Plasma Pharmacokinetics and Tissue Distribution of Bufotalin in Mice Following Single-Bolus Injection and Constant-Rate Infusion of Bufotalin Solution. European Journal of Drug Metabolism and Pharmacokinetics, 35, 115-121. https://doi.org/10.1007/s13318-010-0017-6
Arispe, N., Diaz, J.C., Simakova, O. and Pollard, H.B. (2008) Heart Failure Drug Digitoxin Induces Calcium Uptake into Cells by Forming Transmembrane Calcium Channels. Proceedings of the National Academy of Sciences of the United States of America, 105, 2610-2615.
Bleicher, D.P., Scorup, P.C. and Mitchell, W.W. (1980) Pesticide Use in Alaska, 1978. Agricultural Experiment Station, University of Alaska, Fairbanks.
Couce-Rios, A., Kovacs, G., Ujaque, G. and Lledos, A. (2015) Hydroamination of C-C Multiple Bonds with Hydrazine Catalyzed by N-Heterocyclic Carbene-Gold(I) Complexes: Substrate and Ligand Effects. ACS Catalysis, 5, 815-829. https://doi.org/10.1021/cs501705b
Spízek, J. and Rezanka, T. (2004) Lincomycin, Clindamycin and Their Applications. Applied Microbiology and Biotechnology, 64, 455-464. https://doi.org/10.1007/s00253-003-1545-7
Jones, D.L., Michael, A.M. and Ommer, J.P. (1961) Clinical Trial of Methoserpidine in General Practice. British Medical Journal, 2, 1738-1741.
Hageman, J.H. and Carlton, B.C. (1973) Effects of Mutational Loss of Specific Intracellular Proteases on the Sporulation of Bacillus subtilis. Journal of Bacteriology, 114, 612-617.
Zhao, L., Liu, Z.F., Jin, W.J. and Feng, F. (2021) Luminescence Property of Phosphoramidic Acid Oligomer Nanodots in Aqueous Solution. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 248, Article ID: 119261. https://doi.org/10.1016/j.saa.2020.119261.
Taylor, D.N., Hamer, D.H. and Shlim, D.R. (2017. Medications for the Prevention and Treatment of Travellers’ Diarrhea. Journal of Travel Medicine, 24, S17-S22. https://doi.org/10.1093/jtm/taw097
Emmett, M.R., Bodhuri, P., Zhao, Y., Cammisa, E.G. and Green, S.P. (2020) U.S. Patent No. 10875846B2. U.S. Patent and Trademark Office, Washington DC. https://patents.google.com/patent/US10875846B2/en
Rey, R.A. and Grinspon, R.P. (2020) Androgen Treatment in Adolescent Males with Hypogonadism. American Journal of Men’s Health, 14, Article ID: 1557988320922443. https://doi.org/10.1177/1557988320922443
Surana, A.R. and Wagh, R.D. (2017) GC-MS Profiling and Antidepressant-Like Effect of the Extracts of Hamelia Patens in Animal Model. Bangladesh Journal of Pharmacology, 12, 410-416.
Fesenko, A.A. and Shutalev, A.D. (2018) A General Stereo Selective Approach to 1,2,4-Triazepane-3-Thiones/Ones via Reduction or Reductive Alkylation of 2,4,5,6-Tetrahydro-3H-1,2,4-Triazepine-3-Thiones/Ones. Multidisciplinary Digital Publishing Institute Proceedings, 9, Article No. 14.
Rosier, A., Medeiros, F.H. and Bais, H.P. (2018) Defining Plant Growth Promoting Rhizobacteria Molecular and Biochemical Networks in Beneficial Plant-Microbe Interactions. Plant and Soil, 428, 35-55. https://doi.org/10.1007/s11104-018-3679-5
Magnúsdóttir, S. and Thiele, I. (2018) Modeling Metabolism of the Human Gut Microbiome. Current Opinion in Biotechnology, 51, 90-96. https://doi.org/10.1016/j.copbio.2017.12.005
Gryndler, M., Gryndlerová, H., Hujslová, M., Bystriansky, L., Malinská, H., Simsa, D. and Hrselová, H. (2021) In Vitro Evaluation of Biofilm Biomass Dynamics. Microbiology, 90, 656-665. https://doi.org/10.1134/S0026261721050064
Westerhoff, H.V., Brooks, A.N., Simeonidis, E., García-Contreras, R., He, F., Boogerd, F.C., Jackson, V.J., Goncharuk, V. and Kolodkin, A. (2014) Macromolecular Networks and Intelligence in Microorganisms. Frontiers in Microbiology, 5, Article No. 379. https://doi.org/10.3389/fmicb.2014.00379
Seneviratne, G., Premarathna, M. and Jayasekara, A. (2020) Ecologically Important, a Wider Range of Biochemical Molecules Secreted by Microbial Biofilms Can Give Life and Reinstate Lost Ecosystem Sustainability. National Institute of Fundamental Studies, Kandy. https://doi.org/10.13140/RG.2.2.27873.94565