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Comparing the Effect of Different Concentration of Sulfuric Acid Differentiation Solution on Acid Fast Dyeing
Department of Thoracic, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
- 1 Department of Thoracic, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
- 2 Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
- 3 Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
- 4 Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
Advances in Bioscience and Biotechnology·Volume 13 (2022)·Pages 347–351·Published 8 September 2022·DOI10.4236/abb.2022.139022
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Abstract
Objective: In the acid-fast staining experiment of pathological tissues, sulfuric acid and hydrochloric acid were used to evaluate the staining results, so as to get the best staining method. Methods: Using sulfuric acid differentia tion solution and hydrochloric acid differentiation solution, the paraffin blo cks of pathological tissues known to contain Mycobacterium tuberculosis were compared to evaluate the staining effect. Results: When 0.5% hydrochloric acid differentiation solution is used and the differentiation time is 6 s, the dyeing effect is better than that of sulfuric acid differentiation solution.
KeywordsAcid-Fast DyeingSulfuric AcidDifferentiation SolutionDyeing EffectHydrochloric Acid
- Li, X., Qing, Q. and Li, D.F. (2015) Evaluation of the Effect of Fluorescent Staining and Samarium-Nile Staining in Detecting Mycobacterium Tuberculosis. International Journal of Laboratory Medicine, 36, 745-746.
- Wang, D.T. and Dong, J.Q. (2012) Practical Modern Pathology Technology. Peking Union Medical College Press, Beijing, 133-134.
- Yin, C.L., Zhou, L.R. and Liu, Y. (2016) Introducing an Improved Acid-Fast Bacilli Staining Method. Chinese Journal of Diagnostic Pathology, 23, 554-555.
- Xue, X.W., Wang, D.T., Li, X.Q., et al. (2013) Comparison of Freeze-Drying Effect between Environmental Protection Reagent and Traditional Reagent. Chinese Journal of Diagnostic Pathology, 20, 512 p.
- Kevin, Z, Hu, Z.J. and Zhang, C.L., et al. (2016) Comparison of Basement Membrane Staining Methods and Its Application in Gastric Dysplasia and Gastric Adenocarcinoma. Chinese Journal of Histochemistry and Cytochemistry, 25, 169-173.
- Liang, Y.J., Ling, Q.B. and Wei, Z. (2011) Clinical Pathology Technology. People’s Medical Publishing House, Beijing, 116-118.
- Yang, Q.L. (1991) Synthesis of Diethyl Sulfate by Sulfuric Acid-Ethanol Method. Liaoning Chemical Industry, 22, 22-24.
- Yu, G.Y., Chen, Y.L. and Liao, M.M. (2016) Exploration of the Role of Ethanol in Hydrochloric Acid Ethanol Differentiation Solution. Journal of Practical Medical Technology, 7, 706-707.
- Jie, L.W. (2015) Effect of Improved Acid-Fast Staining Method on the Detection Rate of Acid-Fast Bacilli. Shanxi Medical Journal, 44, 1553-1555.