Isolation and Expression Analysis of Meristem Gene <i>CLV</i>2 and Carpel Generation Gene (<i>CRC</i>) from Multi-Carpel Structure of <i>Thermopsis turcica</i>

Abstract

Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological propert ies . The aim of this study was to determine the factors controlling the interesting feature of this plant. Particularly the controlling features of the flower meristem and the pis tils that mutations in two genes and helps occurring the number of flower organs and can cause abnormalities, This study has attempted to explore the controlling feature of Thermopsis turcica , the young bud Arabidopsis CLV 2, and CRC gene homologs were isolated and analyzed in different tissues. First, with total RNA isolated from the first thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the replicated set of analyses. The full - length cDNA of genes related to RACE analysis was carried out with the sequenc ing process. The real - time PCR method was applied for gene functional analyses with buds, sepals, petal s and stamen and relative expression analysis of genes in the tissue of pistils. Full-length cDNA sequence for the gene CLV 2 2557 bp, the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene —bp, the encoded protein 174 identified as amino acids. Alignment and phyloge netic analyses of CLV 2 and CRC genes were found to be similar to other types of legume s i n homologous set s . Real - time (RT-qPCR) PCR results relative to study courses with genes.