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Molecular Identification and Characterization of a Begomovirus Associated with Okra Enation Leaf Curl Disease in Mali

¹ Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA) de Katibougou, Koulikoro, Mali
² Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA) de Katibougou, Koulikoro, Mali
³ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali
⁴ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali
⁵ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali
⁶ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali
⁷ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali
⁸ Institut Economie Rural (IER), Bamako, Mali
⁹ Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA) de Katibougou, Koulikoro, Mali
¹⁰ Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) de Bamako, Laboratoire de Biologie Moléculaire Appliquée (LBMA), Bamako, Mali

Advances in Bioscience and Biotechnology·Volume 14 (2023)·Pages 429–438·Published 25 October 2023·DOI10.4236/abb.2023.1410029

Abstract

Okra is one of the most widespread vegetable crops in the world, particularly in West Africa. However, several factors influence okra crops as biotic and abiotic factors. Among the diseases affecting its culture, okra leaf curl disease is a major threat. This study aims to assess begomoviruses from okra plants with symptoms like leaves curl by molecular approach. A total of thirteen serologically positive samples were tested by PCR assay, and one sample was sequenced among them. The begomovirus was found in six isolates, Bhendi yellow vein mosaic virus (BYVMV) in three isolates, and Okra enation leaf curl virus (OELCuV) in three isolates, respectively. The begomovirus isolate sequences shared 90% identity with the Cotton leaf curl Gezira virus . Mixed infections between these viruses were found. Thus, these results highlight the need to monitor the spread of these disease-causing viruses for okra crops in Mali. In addition, they can also lead to a considerable loss in okra fields in Koulikoro, which is an agricultural region by excellence.

KeywordsOkraPCRBegomovirusBYVMVOELCuVMali

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Molecular Identification and Characterization of a Begomovirus Associated with Okra Enation Leaf Curl Disease in Mali

Abstract

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