Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas production potential, microbial community and methanogenic metabolic pathways of mixture. Research has shown that mixed fermentation of lignite and straw significantly promoted biomethane production. The abundance of hydrolytic acidifying functional bacteria genera ( Sphaerochaeta , Lentimicrobium ) in mixed fer mentation was higher than that in the fermentation of single lignite and single straw. The abundance of some key CAZy metabolic enzyme gene se quences in mixed fermentation group was increased, which was favorable t o improve methane production. Aceticlastic methanogenesis was the most critical methanogenic pathway and acetic acid pathway was more competitive in methanogenic mode during peak fermentation. Macrogenomics provided theoretical support for the claim that mixed fermentation of coal and straw promoted biomethane metabolism, which was potentially valuable in expanding methanogenesis from mixed fermentation of lignite with different biomasses.
Du, F., Ren, Z.X., Deng, W.A., Li, Z., Wang, X.Z., Wang, H.M. and Fu, X.Y. (2020) Effect of Dry Dewatering on Physicochemical Structure and Hydro-Conversion Performance of Lignite. Journal of China Coal Society, 45, 778-785.
Jin, X.L. (2018) Analysis on Effective Utilization Technology of Lignite. Coal Science and Technology, 46, 260-263, 226.
Scott, A.R. and Kaiser, W.R. (1994) Thermogenic and Secondary Biogenic Gases, San Juan Basin, Colorado and New Mexico—Implications for Coalbed Gas Producibility. AAPG Bulletin, 78, 1186-1209. https://doi.org/10.1306/A25FEAA9-171B-11D7-8645000102C1865D
Ning, J., Zhu, G.F., Lv, N., Wang, X.F., Wang, T., Li, J.J. and Zhou, M.D. (2018) Effects of C/N Ratio on Biogas Production by Anaerobic Co-Digestion of Pig Manure and Corn Straw. Transations of the Chinese Society of Agricultural Engineering, 34, 93-98.
Zhu, X.P., Yellezuome, D., Liu, R.H., Wang, Z.Z. and Liu, X. (2021) Effects of Co-Digestion of Food Waste, Corn Straw and Chicken Manure in Two-Stage Anaerobic Digestion on Trace Element Bioavailability and Microbial Community Composition. Bioresource Technology, 346, Article ID: 126625. https://doi.org/10.1016/j.biortech.2021.126625
Yan, Y.L., Du, Z.W., Zhang, L.Q., Li, F. and Smith, J.A. (2019) Identification of Parameters Needed for Optimal Anaerobic Co-Digestion of Chicken Manure and Corn Stover. RSC Advances, 9, 29609-29618. https://doi.org/10.1039/C9RA05556H
Zhang, D., He, H., Ren, Y., Rizwan, H., Michael, U., Song, J., Ishtiaq, A.M., Asif, J., Adnan, S.M., Guo, H.G., Liu, F.J. and Huang, Z.X. (2022) A Mini Review on Biotransformation of Coal to Methane by Enhancement of Chemical Pretreatment. Fuel, 308, Article ID: 121961. https://doi.org/10.1016/j.fuel.2021.121961
Pan, Y.X., Liu, S.Q. and He, Y.Q. (2020) Methane Production Performance of Semi-Continuous Anaerobic Fermentation with Rice Straw Pretreated by Microbial Community. Transations of the Chinese Society of Agricultural Engineering, 36, 261-266.
Dong, X.L., Lin, Q.S. and Lin, Y.N. (2022) Effects of Electrolytes on Acidogenic Fermentation of Waste Activated Sludge for Volatile Fatty Acids Production via Electrochemical Pretreatment. Environmental Engineering, 40, 71-78.
Xia, D.P., Chen, X., Wang, C. and Su, X.B. (2017) Experimental Study on the Production of H2-CH4 from Lignite Jointly with Acid-Alkali Pretreatment-Microbial Gasification. Journal of China Coal Society, 42, 3221-3228.
Haider, R., Ghauri, M.A., Sanfilipo, J.R., Jones, E.J., Orem, W.H., Tatu, C.A. and Akhtar, N. (2013) Fungal Degradation of Coal as a Pretreatment for Methane Production. Fuel, 104, 717-725. https://doi.org/10.1016/j.fuel.2012.05.015
Guo, H.G., Chen, Q.L., Hu, H.W. and He, J.Z. (2020) High-Solid Anaerobic Co-Digestion of Pig Manure with Lignite Promotes Methane Production. Journal of Cleaner Production, 258, Article ID: 120695. https://doi.org/10.1016/j.jclepro.2020.120695
Yoon, S.P., Jeon, J.Y. and Lim, H.S. (2016) Stimulation of Biogenic Methane Generation from Lignite through Supplying an External Substrate. International Journal of Coal Geology, 162, 39-44. https://doi.org/10.1016/j.coal.2016.05.009
Guo, H.Y., Dong, Z.W., Su, X.B., Liu, S., Jia, J.B., Yu, H.F. and Xia, D.P. (2018) Synergistic Biodegradation of Coal Combined with Corn Straw as a Substrate to Methane and the Prospects for Its Application. Energy Fuels, 32, 7011-7016. https://doi.org/10.1021/acs.energyfuels.8b01120
Guo, H.Y., Zhang, M.L., Dong, Z.W., Wang, Q., Xia, D.P., Lv, J.H. and Yu, H.F. (2019) The Mechanisms of Biogenic Methane Metabolism by Synergistic Biodegradation of Coal and Corn Straw. Bioresource Technology, 298, Article ID: 122577. https://doi.org/10.1016/j.biortech.2019.122577
Orellana, E., Davies-Sala, C., Guerrero, L.D., Vardé, I., Altina, M., Lorenzo, M.C., Figuerola, E.L., Pontiggia, R.M. and Erijman, L. (2019) Microbiome Network Analysis of Co-Occurrence Patterns in Anaerobic Co-Digestion of Sewage Sludge and Food Waste. Water Science & Technology, 79, 1956-1965. https://doi.org/10.2166/wst.2019.194
He, Q., Li, L. and Zhao, X.F. (2022) Metagenomic Analysis of Microbial Characteristics in Over-Acidification Systems during Anaerobic Digestion of Food Waste. Acta Scientiae Circumstantiae, 42, 374-384.
Luo, X. (2015) Research and Application of Metagenomic Classification and Analysis Method. Master’s Thesis, Southeast University, Nanjing.
Su, X.B., Zhao, W.Z. and Xia, D.P. (2018) The Diversity of Hydrogen-Producing Bacteria and Methanogens within an in Situ Coal Seam. Biotechnol. Biotechnology for Biofuels, 11, Article No. 245. https://doi.org/10.1186/s13068-018-1237-2
Su, X., Wang, J.H. and Zhang, F.Z. (2020) Microbial Community Succession Associated with Corn Straw Degradation in a Bacterium Consortium. Acta Microbiologica Sinica, 60, 2675-2689.
Hahnke, R.L., Meier-Kolthoff, J.P. and García-López, M. (2018) Genome-Based Taxonomic Classification of Bacteroidetes. Frontiers in Microbiology, 9, Article 354825. https://doi.org/10.3389/fmicb.2018.00304
Dong, X.Y., Greening, C., Brüls, T., Conrad, R., Guo, K., Blaskowski, S., Kaschani, F., Kaiser, M., Laban, N.A. and Meckenstock, R.U. (2018) Fermentative Spirochaetes Mediate Necromass Recycling in Anoxic Hydrocarbon-Contaminated Habitats. The ISME Journal, 12, 2039-2050. https://doi.org/10.1038/s41396-018-0148-3
Dai, Z.M., Su, W.Q., Chen, H.H., Barberán, A., Zhao, H.C. and Yu, M.J. (2018) Long-Term Nitrogen Fertilization Decreases Bacterial Diversity and Favors the Growth of Actinobacteria and Proteobacteria in Agro-Ecosystems across the Globe. Global Change Biology, 24, 3452-3461. https://doi.org/10.1111/gcb.14163
Miyazaki, M., Sakai, S. and Ritalahti, K.M. (2014) Sphaerochaeta multiformis sp. nov., an Anaerobic, Psychrophilic Bacterium Isolated from Subseafloor Sediment, and Emended Description of the Genus Sphaerochaeta. International Journal of Systematic and Evolutionary Microbiology, 64, 4147-4154. https://doi.org/10.1099/ijs.0.068148-0
Liu, G.H., Wang, J. and Qi, L. (2022) Study on Regulating Anaerobic Fermentation for Producing Short-Chain Fatty Acids from Primary Sludge in WWTPs by Different Bio-Enzymes. China Environmental Science, 42, 2195-2203.
Zhang, L. and Loh, K.C. (2019) Synergistic Effect of Activated Carbon and Encapsulated Trace Element Additive on Methane Production from Anaerobic Digestion of Food Wastes—Enhanced Operation Stability and Balanced Trace Nutrition. Bioresource Technology, 278, 108-115. https://doi.org/10.1016/j.biortech.2019.01.073
Thommes, M., Katsumi, K. and Alexander, V. (2015) Physisorption of Gases, with Special Reference to the Evaluation of Surface Area and Pore Size Distribution (IUPAC Technical Report). Pure and Applied Chemistry, 87, 1051-1069. https://doi.org/10.1515/pac-2014-1117
Chen, Y., Luo, J. and Yan, Y. (2013) Enhanced Production of Short-Chain Fatty Acid by Co-Fermentation of Waste Activated Sludge and Kitchen Waste under Alkaline Conditions and Its Application to Microbial Fuel Cells. Applied Energy, 102, 1197-1204. https://doi.org/10.1016/j.apenergy.2012.06.056
Najmudin, S., Guerreiro, C.I. and Carvalho, A.L. (2006) Xyloglucan Is Recognized by Carbohydrate-Binding Modules That Interact with β-Glucan Chains. Journal of Biological Chemistry, 281, 8815-8828. https://doi.org/10.1074/jbc.M510559200
Vanwonterghem, I., Jensen, P.D. and Rabacy, K. (2016) Genome-Centric Resolution of Microbial Diversity, Metabolism and Interactions in Anaerobic Digestion. Environmental Microbiology, 18, 3144-3158. https://doi.org/10.1111/1462-2920.13382
Hanreich, A., Schimpf, U. and Zakrzewski, M. (2013) Metagenome and Metaproteome Analyses of Microbial Communities in Mesophilic Biogas-Producing Anaerobic Batch Fermentations Indicate Concerted Plant Carbohydrate Degradation. Systematic and Applied Microbiology, 36, 330-338. https://doi.org/10.1016/j.syapm.2013.03.006
Cantarel, B.L., Coutinho, P.M. and Corinne, R. (2009) The Carbohydrate-Active EnZymes Database (CAZy): An Expert Resource for Glycogenomics. Nucleic Acids Research, 37, 233-238. https://doi.org/10.1093/nar/gkn663
Zheng, M.J., Li, X.Y. and Li, L. (2013) Homology Modeling of Glucosyltransferases from Pueraria lobata (Willd.) Ohwi and the Analysis of the Active Motif. China Journal of Bioinformatics, 11, 287-292.
Stam, M.R., Danchin, E.G.J. and Rancurel, C. (2006) Dividing the Large Glycoside Hydrolase Family 13 into Subfamilies: Towards Improved Functional Annotations of alpha-Amylase-Related Proteins. Protein Engineering, Design and Selection, 19, 555-562. https://doi.org/10.1093/protein/gzl044
Yin, Q., Yang, S. and Wang, Z. (2018) Clarifying Electron Transfer and Metagenomic Analysis of Microbial Community in the Methane Production Process with the Addition of Ferroferric Oxide. Chemical Engineering Journal, 333, 216-225. https://doi.org/10.1016/j.cej.2017.09.160
Thapa, L.P., Lee, S.J. and Park, C. (2017) Production of L-Lactic Acid from Metabolically Engineered Strain of Enterobacter aerogenes ATCC 29007. Enzyme and Microbial Technology, 102, 1-8. https://doi.org/10.1016/j.enzmictec.2017.03.003
Zhang, M.L., Guo, H.Y. and Xia, D.P. (2022) Metagenomic Insight of Corn Straw Conditioning on Substrates Metabolism during Coal Anaerobic Fermentation. Science of the Total Environment, 808, Article ID: 152220. https://doi.org/10.1016/j.scitotenv.2021.152220