The Combined Effect of Lumenato and Ceramide in the Protection of Collagen Damage Induced by Neutrophils in Normal Human Dermal Fibroblasts

Abstract

Introduction : Collagen is the primary structural protein fibroblasts produce in the skin’s extracellular matrix. Infiltration of neutrophils into the epidermis and dermis by exposure to UV causes collagen damage and contributes to photoaging. Methods : To study the combined effect of Lumenato and ceramide in preventing collagen-1 damage induced by phagocytes, we used co-cultures o f normal human dermal fibroblasts (fibroblasts) and activate d human neutrophils. The present study aimed to determine the protective effect of the combination of Lumenato and ceramide on fibroblast collagen-1 damage induced by neutrophils. Results: Lumenato (in the range of 6.5 - 208 μg/ml) or ceramide (in the range of 0.1 - 50 μM) inhibited the production of superoxides and MPO by TNF α -stimulated neutrophils, as well as the production of NO by LPS-stimulated macrophages in a dose-dependent manner. The combinations of Lumenato and ceramide, in low concentrations, caused synergis tic prevention of fibroblasts’ collagen-1 damage induced by TNF α -activate d n eutrophils, detected by fluorescence immunostaining and WB analys is. MPO activity in the supernatants of the co-cultures was also synergistically inhibited. Adding Lumenato or ceramide singly or in combinations in these low concentrations to the fibroblast cultures did not affect the expression of collagen-1. The combinations of Lumenato or ceramide in these concentrations al so caused a synergistic inhibition of NO production by activated macro phages. Conclusions : The results suggest that combining low concentrations o f Lumenato and ceramide results in synergistic protection against fibro blasts’ collagen-1 damage induced by neutrophils, thus indicating their possible potential for enhanced skin health.