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Sensory Phenotypic and Molecular Identification of Aromatic Rice Accessions Cultivated in Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Biotechnologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Plant, Horticultural and Forestry Sciences, National University of Agriculture, Kétou, Benin
Genetics Teaching and Research Unit, Department of Biochemistry-Genetics, Peleforo GON COULIBALY University, Korhogo, Côte d’Ivoire
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Plant, Horticultural and Forestry Sciences, National University of Agriculture, Kétou, Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 1 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Biotechnologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 2 Laboratory of Plant, Horticultural and Forestry Sciences, National University of Agriculture, Kétou, Benin
- 3 Genetics Teaching and Research Unit, Department of Biochemistry-Genetics, Peleforo GON COULIBALY University, Korhogo, Côte d’Ivoire
- 4 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 5 Laboratory of Plant, Horticultural and Forestry Sciences, National University of Agriculture, Kétou, Benin
- 6 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 7 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 8 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 9 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
- 10 Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National School of Applied Biosciences and Bio-technologies, National University of Science, Technology, Engineering and Mathematics, Dassa-Zoumè, Benin
Advances in Bioscience and Biotechnology·Volume 15 (2024)·Pages 195–206·Published 14 March 2024·DOI10.4236/abb.2024.153014
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Abstract
Rice is one of the most widely cultivated cereals in the world, and its aroma is increasingly in demand. With the advancement of research, a major rice fla vor gene has been identified on rice chromosome 8. It encodes non-functional betaine aldehyde dehydrogenase leading to the accumulation of 2-acetyl-1-pyrroline which is the major olfactory compound that confers the fragrant character to rice. The aroma of rice is considered a special trait of enormous economic importance that determines the prime price in world trade. To satisfy the needs of the population and reduce rice imports into Benin, we conducted this study to identify aromatic rice accessions grown in Benin. Seventy-two rice accessions collected across Benin were PCR amplified with three SSR markers RM 7049, Aro 7, and RM 223, linked to the fgr (fragrance of rice) aroma gene. Molecular analysis revealed that 12 of the 72 accessions, namely Bagou 19, Bagou 22, Tchaka 34, Foun 15, Tchaka 41, Nana 32, Kan 61, Kung 69, Kung 67, Bagou 20, Agbab 101 and Koum 55 possess the fgr gene and can be considered as aromatic rice accessions. A sensory phenotypic test using KOH was carried out on rice accessions carrying fgr gene. Of the twelve positives, only one had the smell of aromatic rice, like the Azucena control. These results show that Benin also has aromatic rice varieties that can be sold on national and international markets.
KeywordsAromatic Rice2-Acetyl-1-PyrrolineSSR MarkersBenin
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