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Evaluation of the Polymorphic Locus rs1695 of the GSTP1 Gene Association with HPV Infection in Women in Burkina Faso

¹ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
² Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
³ National Centre for Scientific and Technological Research (CNRST)/National Centre for Scientific and Technological Research (CNRST), Ouagadougou, Burkina Faso
⁴ National Centre for Scientific and Technological Research (CNRST)/National Centre for Scientific and Technological Research (CNRST), Ouagadougou, Burkina Faso
⁵ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
⁶ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
⁷ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
⁸ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
⁹ University Post Office, Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana
¹⁰ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹¹ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹² Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹³ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹⁴ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹⁵ Higher School of Biological and Food Techniques (ESTBA), University of Lomé, Lomé, Togo
¹⁶ Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
¹⁷ Morphology and Organogenesis Laboratory (LAMO), Université Joseph KI-ZERBO, UFR/SDS, Ouagadougou, Burkina Faso

Advances in Bioscience and Biotechnology·Volume 16 (2025)·Pages 167–177·Published 16 May 2025·DOI10.4236/abb.2025.165010

Abstract

Introduction: Genetic polymorphisms of certain classes of glutathione S-transferase (GST), the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several cancers such as cervical cancer. The objective of this study was to characterize the impact of the rs1695 polymorphism of GSTP1 in women infected by high-risk human papillomavirus (HR-HPV) in Ouagadougou, Burkina Faso. Methods: Genotyping of GSTP1 rs1695 polymorphisms was performed in 55 women with high-risk HPV infection, and 89 healthy controls using the PCR-RFLP method. Conventional PCR was used for DNA amplification and the enzymes Alw26I or BsmA1 were used for enzymatic digestion. Results: The prevalence of GSTP1 rs1695 AA, AG and GG genotypes was respectively 27.8%, 45.8% and 26.4% in the study population with a mutation rate of 49.31%. However, the frequency of AA, AG and GG genotypic was respectively 30.3%, 45%, 24.7% in controls and 23.6%, 47.3%, 29.1% in cases. Conclusion: Our study allowed us to characterize the frequencies of GSTP1 rs1695 genotypes in the study population, in cases and controls. From our analysis, none of the three genotypes of GSTP1 rs1695 weren’t associated with a risk or a protective factor for HR-HPV infection in women in Burkina Faso.

KeywordsPolymorphismGSTP1WomenHPVBurkina Faso

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Evaluation of the Polymorphic Locus rs1695 of the GSTP1 Gene Association with HPV Infection in Women in Burkina Faso

Abstract

References (26)