Effect of intraperitoneal and intramuscular injection of killed aeromonas hydrophila on lymphocytes and serum proteins of common carp, cyprinus carpio
- 1
- 2
- 3
- 4
Abstract
The effects of injectable killed Aeromonas hydrophila on lymphocyte populations and on serum proteins of juvenile common carp, Cyprinus carpio, were examined. The fishes were injected either intraperitoneally or intramuscularly with killed A. hydrophila bacterin isolated from a diseased fish. 15 days after injection the lymphocyte count was significantly higher in the kidney of intraperitoneally bacteria injected fishes than in the normal saline injected fishes (p < 0.05), but in the blood smears the lymphocyte count difference was not significant (p > 0.05). In the intramuscular bacteria injected fishes in comparison with the normal saline group, the lymphocyte count was significantly higher only in the blood (p < 0.05) but in the kidney the difference was not significant (p > 0.05). In comparison between the serum protein parameters in different groups, in intraperitoneal injection of killed-bacteria, total albumin as well as the ratio of albumin/globulin was greater than the control group (p < 0.05). The other fractions had not showed any significant difference (p > 0.05). In intramuscular injection, the normal saline-injected group, amount of α?-globulin was significantly higher than bacteria-injected group (p < 0.05). For the other fractions there was no significant difference between two groups (p > 0.05).
- Austin, B. and Adams, C. (1996) Fish pathogens. In: Austin, B., Altwegg, M., Gosling, P.J. and Joseph, S. (Eds) The Genus Aeromonas, John Wiley and Sons, Chichester, pp. 197-243.
- Nielsen, M.E. Schmidt, A. Qian, D. Shimada, T. Shen, J.Y. and Larsen, J.L. (2001) Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang province of China. Disease of Aquatic Organisms, 46, 23-29.
- Roberts, R.J. (1997) Epizootic ulcerative syndrome (EUS): progress since 1985. In: Flegel T.W. and MacRae I.H. (Eds) Diseases in Asian aquaculture III. Asian Fisheries Society, Manila, pp. 125-128
- Ansary, A., Haneef, R.M., Torres, J.L. and Yadav, M. (1992) Plasmids and antibiotic resistance in Aeromonas hydrophila, isolated in Malaysia from healthy and diseased fish. Journal of Fish Diseases, 15, 191-196.
- Fang, H.M., Ling, K.C., Ge, R. and Sin. Y.M. (2000) Enhanchment of productive immunity in blue gourami, Trichogaster trichopterus (Pallas), against Aeromonas hydrophila and Vibrio anguillarum by A. hydrophila major adhesion. Journal of Fish Diseases. 23, pp. 137-145.
- Loghothetis, P.N. and Austin, B. (1994) Immune response of rainbow trout (Oncorhynchus mykiss, Walbaum) to Aeromonas hydrophila. Fish and Shell Fish Immunology, 4(23), 254.
- Baba, T., Imamura, J. and Izawa, K. (1988) Immune protection in carp, Cyprinus carpio L., after immunization with Aermonas hydrophila crude lipopolysaccharide. Journal of Fish Diseases, 11, 237-244.
- Stevenson, R.M.W. (1988) Vaccination against Aeromonas hydrophila. In: Ellis, A.E. (Ed.), Fish Vaccination, Academic Press, London, pp. 112-123.
- Kozinska, A. and Guz, L. (2004) The effect of various Aeromonas bestiarum vaccines on non-specific immune parameters and protection of carp (Cyprinus carpio L.). Fish and Shellfish Immunology, 16, 437-445.
- Magnadottir, B. and Gudmundsdottir, B.K. (1992) A composition of total and specific immunoglobulin levels in healthy Atlantic salmon (Salmo salar, L.) and in salmon naturally infected with Aeromonas salmonicida sub sp. Veterinary Immunology and Immunopathology, 32, 179-189.
- Moyner, K. (1993) Changes in serum protein composition occur in Atlantic salmon, Salmo salar L. during Aeromonas salmonicida infection. Journal of Fish Diseases. 16: 6. 601-604.
- Evenberg, D., de Graaff, P., Fleuren, W. and van Muiswinkel, W.B. (1986) Blood changes in carp (Cyprinus carpio) induced by ulcerative Aeromonas salmonicida infections. Veterinary Immunology and Immunopathology, 12, 321-330.