The JAR choriocarcinoma cell line share many of the characteristics of early placental trophoblast cells including the invasion properties. Matrix metallo-proteinases (MMPs), the main actors of matrix proteolysis, are involved in normal invasion as well as in the invasive character of tumor cells and the metastase formation. Tumor necrosis factor- α (TNF- α ) is present in the placental environment and TNF- α levels are elevated in some placental pathologies. In the present work, we addressed whether TNF- α is a modulator of JAR cell MMP secretion. Following TNF- α stimulation, zymographic analysis showed the increased secretion of the active form of MMP-2 and to a lesser extent proMMP-2 and MMP-9. In addition, MMP-2 gene expression only increased slightly whereas MMP-9 and TIMP-1 transcripts were undetectable. This suggests that TNF- α may modulate the secretion of MMPs independently of MMP gene expression control.
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