The nature of adhesion factors which lie on the surfaces of <i>Lactobacillus</i> adhering to cells

Abstract

Lactobacillus adheres to intestinal epithelial cells and yeast fungus cells with the aid of adhesion factors expressed on its cell surface. To identify adhesion factors nature on the surface of Lactobacillus , an adhesion experiment was carried out by pre-treating the Lactobacillus supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10min. Additionally, intestinal epithelial cells were treated with sodium iodate, trypsin and sugar inhibition tests to characterize the receptors in Lactobacillus that interact with intestinal epithelial cells. It was demonstrated that Lactobacillus adhesion ability was decreased (P < 0.01) after treating the supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10 min respectively. The adhesion factor on the surface of Lactobacillus cells was identified as a D-mannose glycoprotein. This observation was confirmed after treating intestinal epithelial cells with sodium iodate and trypsin, and sugar inhibition tests. Wild type Lactobacillus can agglutinate yeast fungus cells but after being exposured to mannose, agglutination to yeast fungus cells is lost or reduced. Results from this study we also got that inactivated and live bacteria that similarly adhere to intestinal epithelial cells.