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Interferon-gamma ELISPOT for the screening and diagnosis of latent tuberculosis infection in healthy population of China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Guangdong Provincial Center for Tuberculosis Control, Guangzhou, China
Baoan Chronic Diseases Prevention and Cure Hospital, Shenzhen, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Baoan Chronic Diseases Prevention and Cure Hospital, Shenzhen, China
Guangdong Provincial Center for Tuberculosis Control, Guangzhou, China
Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 1 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 2 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 3 Guangdong Provincial Center for Tuberculosis Control, Guangzhou, China
- 4 Baoan Chronic Diseases Prevention and Cure Hospital, Shenzhen, China
- 5 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 6 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 7 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 8 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
- 9 Department of Microbiology, Ministry of Education Key Laboratory of Tropical Diseases Control, Gangdong Provincial Department of Education Key Laboratory of Functional Molecules from Marine Microorganisms, Gangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Advances in Bioscience and Biotechnology·Volume 03 (2012)·Pages 259–268·Published 21 June 2012·DOI10.4236/abb.2012.33036
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Abstract
The aim of this study was to analyze the combination of three kinds of in-house IFN- γ ELISPOT using peptide A53 and peptide mixtures (E6 + E7 and E6 + E7 + C14) with tuberculin skin test (TST) to detect latent TB infection (LTBI) in China. A total of 788 healthy people were recruited and analyzed by three kinds of IFN-g ELISPOT, 581 of them had TST results, of which 147 samples were also compared with the T- SPOT.TB test. The positive detection rates for T- SPOT.TB and three kinds of IFN- γ ELISPOT with A53, E6 + E7 and E6 + E7 + C14 were 14.28% (21/147), 29.43% (171/581), 23.24% (135/581) and 28.40% (165/581), respectively. These results were significantly lower than the positive TST results, which were positive in 82.99% (122/147) and 75.73% (440/ 581), respectively. The positive detection rates of three kinds of IFN- γ ELISPOT (31.60%, 26.65% and 32.11% in 788 cases, respectively) could better reflect over 40.00% of Mycobacterium tuberculosis (MTB) infection rate in China. Detection rates between contacts and non-contacts by three kinds of IFN- γ ELIS-POT were not significantly different (p > 0.05). It can be seen that the three kinds of in-house IFN- γ ELIS- POT might be used as a complementary tool of T- SPOT.TB for detecting LTBI in the healthy population of China.
KeywordsLatent TB Infection (LTBI)DetectionIFN-<i>γ</i>ELISPOTHealthy Population of China
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