Extraction, purification and identification of surface adhesion ligand from <i>Lactobacillus</i>
- 1 College of Animal Science & Technology, Shandong Agriculture University, Tai’an, China
- 2 College of Animal Science & Technology, Shandong Agriculture University, Tai’an, China
- 3 College of Animal Science & Technology, Shandong Agriculture University, Tai’an, China
- 4 College of Animal Science & Technology, Shandong Agriculture University, Tai’an, China
Abstract
The objective of this experiment was to purify and analyze adhesion protein from the surface of Lactobacillus . The surface adhesion ligand of Lactobacillus was isolated with of LiCl method and (NH 4 ) 2 SO 4 method, followed by sephadex chromatography, analyzed with discontinuous native polyacrylamide gel electrophoresis, and tested with a binding assay with intestinal epithelial cells. We get the adhesion matter by two methods: ammonium sulfate and LiCl extraction. Further purification with sephadex chromatography produced 3 components. The second component of eluted from chromatography had adhesion enhancing ability compared with the control. A single band was present in discontinuous native—poly-acrylamide gel electrophoresis analysis and molecular weight was determined to be 43-66 KDa. The purified adhesion ligand can improve the number of Lactobacillus adhering to the intestinal epithelial cells.
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