Characterization of Exo 1, 4-<i>β</i> glucanase produced from <i>Tricoderma Viridi</i> MBL through solid-state bio-processing of orange peel waste
- 1 Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan
- 2 Department of Biochemistry, NSMC University of Gujrat, Pakistan
- 3 Department of Biochemistry and Molecular Biology, University of Gujrat, Pakistan
Abstract
Agro-industrial residues are primarily composed of complex polysaccharides that strengthen the microbial growth for the production of industrially important enzymes like cellulases. In the present study we aimed to characterize the Exo 1, 4- β glucanase that was indigenously produced from Trichoderma viride MBL. T. viride MBL was cultured in the Solid-State medium of orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity of 412 ± 12 U/mL was recorded after 4 th day of incubation at pH 5.5 and 30℃. Exo 1, 4- β glucanase was 4.17-fold purified with specific activity of 642 U/mg in comparison to the crude extract. To confirm its purity and molecular weight, sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS- PAGE) was performed. The enzyme was shown to have a molecular weight of 60 kDa with an optimum pH and temperature of 5 and 50℃, respectively. Lineweaver-Burk reciprocal plot revealed that the kinetic constants K m and V max of purified Exo 1, 4- β glucanase were 76 μM and 240 U/mL.
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