Effects of <i>Baicalin</i> and <i>Ligustrazine</i> on airway inflammation and remodeling and underlying mechanism in asthmatic rats
- 1 Respiratory department, Tongji hospital, Tongji medical college, Huazhong University of science and technology, Wuhan, Hubei province, China
- 2 Respiratory Department, The First Hospital of Shanxi Medical University, Taiyuan, China
- 3 Respiratory department,the first hospital of Shanxi medical university, Taiyuan, Shanxi province, China
- 4 Respiratory department,the first hospital of Shanxi medical university, Taiyuan, Shanxi province, China
- 5 Respiratory department,the first hospital of Shanxi medical university, Taiyuan, Shanxi province, China
- 6 Respiratory department, Tongji hospital, Tongji medical college, Huazhong University of science and technology, Wuhan, Hubei province, China
Abstract
Aim: To explore the effects of Baicalin and Ligustrazine on airway inflammation and construction and underlying mechanisms through the expressions of GATA-3, IL-5, MMP-9 and TIMP-1 in asthmatic rats. Methods: 30 Wistar rats were randomly divided equally into five groups. Lung tissues were sliced. WBC and Eos in lung tissue were estimated by HE stain and the expressions of IL-5, GATA-3, MMP-9, TIMP-1 and collagen type IV in lung tissue were observed by immunohistochemistry. The airway wall and airway smooth muscle thicknesses were measured by computed image analysis system. Results: Compared with asthma group, EOS counts and the expression of IL-5 and GATA-3 in the lung tissue were significantly lower in normal controlled groups (P < 0.05 or P < 0.01). Additionally, the thickness of airway wall and airway smooth muscle did significantly increase, and the expressions of collagen type Ⅳ, MMP-9 and TIMP-1 were significantly higher in asthma group (P < 0.05). With the intervention of Baicalin or Ligustrazine , EOS decreased, and the thicknesses of airway wall and airway smooth muscle became thinner compared with asthma group. Meanwhile, the expression of collagen type IV, IL-5, GATA-3, MMP-9 and TIMP-1 significantly decreased (P < 0.05). Airway wall thickness and collagen type Ⅳ were associated with Eos, IL-5, TAGA-3, MMP-9, TIMP-1 and MMP-9/ TIMP-1. Conclusion: Two herbs could diminish infiltration of EOS with inhibiting the expressions of IL-5, and GATA-3, meanwhile, decrease the deposition of collagen type IV and the thickness of the airway smooth muscle through regulating MMP-9, TIMP-1 level and the balance between MMP-9 and TIMP-1, additionally, had synergetic effects.
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