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Effect of a rotating frame on preventing bead aggregation in a microfluidic device
Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375
Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011
- 1 Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011
- 2 Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375
- 3 Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011
Advances in Bioscience and Biotechnology·Volume 03 (2012)·Pages 603–608·Published 21 September 2012·DOI10.4236/abb.2012.35078
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Abstract
Varying bead concentrations over the course of experiments have been reported by many scientists. A new device was designed and fabricated to eliminate bead aggregation in a syringe pump prior to flowing through a microchannel. We have molded the effects of rotation in the absence of longitudinal flow by evenly populating the cross section of a syringe with particles, then tracking their movement due to rotation, gravity, and centripetal forces. We have shown both experimentally and numerically that the concentration of the beads remains constant over the course of experiments once the rotational device is used. However, the concentration of the beads drops significantly once no rotation is applied during the experiment.
KeywordsBead AggregationMicrofluidicsRotating Frame
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