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A novel strain of <i>Trichoderma viride</i> shows complete lignocellulolytic activities
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 1 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 2 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 3 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 4 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 5 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 6 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 7 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 8 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 9 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
- 10 Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram, India
Advances in Bioscience and Biotechnology·Volume 03 (2012)·Pages 1160–1166·Published 27 December 2012·DOI10.4236/abb.2012.38142
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Abstract
In this study we describe a novel dark-green strain of Trichoderma viride exhibiting complete ensemble of cellulase, hemicellulase and ligninase activities on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was fortified with synthetic (carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose) and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO 3 or NH 4 NO 3 ) sources. Temperature and pH optima were 28°C and 4, respectively for the growth of the fungus in CMC-BSM with 137 U/mL cellulase activity, which was enhanced to 173 U/mL at 1.25% CMC concentration. Flours of potato and banana peel supported comparable yields of cellulase to that of CMC, while sodium nitrate was the preferred nitrogen source. The water soluble bluish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 292 nm. To sum up, the complete lignocellulolytic potential of this fungus offers great industrial significance, coupled with the production of a new pigment.
Keywords<i>Trichoderma viride</i>MorphologyLiquid FermentationBluish-Green Pigment
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