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Determination of amino-acidic positions important for <i>Ocimum basilicum</i> geraniol synthase activity
Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
Institut de Biologie Moléculaire des Plantes, Université de Strasbourg, Strasbourg, France
Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
- 1 Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
- 2 Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
- 3 Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
- 4 Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
- 5 Institut de Biologie Moléculaire des Plantes, Université de Strasbourg, Strasbourg, France
- 6 Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France
Advances in Bioscience and Biotechnology·Volume 04 (2013)·Pages 242–249·Published 28 February 2013·DOI10.4236/abb.2013.42033
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Abstract
Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum . The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation.
KeywordsGeraniol SynthaseHeterologous ExpressionMonoterpenolsPoint MutationsYeast
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