N-methyl-D-aspartate (NMDA) mediates vascular relaxation via nitric oxide (NO) in rats but not in mice
- 1
Abstract
Amperometric studies have indicated that substance P as well as NMDA stimulates release of NO in rat aortic rings. These data have been confirmed by functional observations of vaso-relaxant action of NMDA within noradrenaline pre-contracted aortic rings, supporting the presence of NMDA receptor in rat aortic rings. It is known that the enzyme endothelial NO synthase (eNOS) mediates vasodilatation not only in rats, but also in C57BL6 mice aortic ring, indicating that in this blood vessel NO is the endogenous endothelium-derived vasodilator. In this work, amperometry together with specifically nitrites insensitive micro-biosensors have been applied to examine the effect of NMDA and substance P upon NO release in rat and in two strains of mice aortic rings. The electrochemical data monitored demonstrate that NMDA mediates vascular relaxation via NO in rats but not in mice. These results are supported by functional data, therefore they suggest that NMDA receptors are “not responding” within these experimental conditions in mice aortic rings.
- Luo, D., Knezevich, S. and Vincent, S. (1993) NMDA induced NO release: An in vivo microdialysis study. Neuroscience, 57, 897-900.
- Crespi, F. (1990) In vivo voltammetry with microbiosensor for analysis of neurotransmitter release and metabolism. Journal of Neuroscience Methods, 34, 53-658.
- Crespi, F., Lazzarini, C., Andreoli, M. and Vecchiato, E. (2000) Voltammetric and functional evidence that N-methyl-D-aspartate and substance P mediate rat vascular relaxation via nitrogen monoxide release. Neuroscience Letters, 287(3), 219-222.
- Crespi, F., Vecchiato, E., Lazzarini, C. and Gaviraghi, G. (2001) Electrochemical evidence that lacidipine stimulates release of nitrogen monoxide (NO) in rat aorta. Neuroscience Letters, 298(3), 171-174.
- Huang, P.L., Huang, Z., Mashimo, H., Bloch, K.D., Moskowitz, M.A. and Fishman, M.C. (1995) Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature, 377(21), 239-242.
- Chataigneau, T., Feletou, M., Huang, P.L., Fishman, M.C., Duhault, J. and Vanhoutte, P.M. (1999) Acetylcholine-induced relaxation in blood vessels from endothelial nitric oxide synthase knockout mice. British Journal of Pharmacology, 126(1), 219-226.
- Furchgott, R.F. (1980) The obbligatory role of endothelial cells in the relaxation of arterial smooth muscle by Acetylcholine. Nature, 288, 373-376.
- Privat, C., Lantoine, F., Bedioui, F., Millanvoye, van Brussel, E., Devynck, J. and Devynck, M.A. (1997) Nitric Oxide production by endothelial cells: comparison of three methods of quantification. Life Sciences, 61(12), 1193-1202.
- Crespi, F., Martin, K.F. and Marsden, C.A. (1988) Measurement of extracellular basal levels of serotonin in vivo using nafion coated carbon fibre microelectrodes combined with differential pulse voltammetry. Neuroscience, 27, 885-898.
- Crespi, F. (2009) Anxiolytics antagonize Yohimbine- induced central noradrenergic activity: a concomitant in vivo voltammetry-electrophysiology model of anxiety. Journal of Neuroscience Methods, 180, 97-105.
- Friedemann, M.N., Robinson, S.W. and Gerhardt, G.A. (1996) o-Phenylenediamine-Modified Carbon Fiber Electrodes for the Detection of Nitric Oxide. Analitical Chemistry, 68, 2621-2628.
- Oldham, C.D., Li, C., Feng, D., Scott, R.O., Wang, W.Z., Moore, A.B., Girard, P.R., Huang, J., Caldwell, R.B., Caldwell, R.W. and May, S.W. (1997) Amidative peptide processing and vascular function. American Journal of Physiology, 273, 1908-1914.