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PCR-DGGE analysis of earthworm gut bacteria diversity in stress of <i>Escherichia coli</i> O157:H7
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, USA
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
- 1 College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
- 2 Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, USA
- 3 College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
- 4 College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
- 5 College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
- 6 College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
Advances in Bioscience and Biotechnology·Volume 04 (2013)·Pages 437–441·Published 29 March 2013·DOI10.4236/abb.2013.43A058
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Abstract
In order to test if the intestinal bacteria play an important role in antibacterial ability of earthworm, we chose Escherichia coli O157:H7, an anthropozoonosis pathogen, as a biological stressor and studied the cha nge of intestinal bacteria community of earthworm by PCR-DGGE analysis. Results showed that the pathogen merely existed 1 - 3 days, then almost disappeared after through the earthworm’s gut. In this period, the diversity and abundance index of intestinal bacteria increased first, then decreased, and final ly kept stably after 7 days. The result demonstrat ed that the intestinal bacteria of earthworm had ability of adjust community structure to eliminate the pathogen E. coli O157:H7, and the amount of bacteria Bacillus increased significantly, which might be the positive antagonism to E. coli O157:H7.
KeywordsPCR-DGGEEarthworm Intestinal Bacteria Diversity<i>Escherichia coli</i>O157:H7 Stress
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