Metabolic regulation of <i>Escherichia coli</i> cultivated under anaerobic and aerobic conditions in response to the specific pathway gene knockouts — Oak Academic Publishing
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Metabolic regulation of <i>Escherichia coli</i> cultivated under anaerobic and aerobic conditions in response to the specific pathway gene knockouts
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Fukuoka, Japan
,
Institute of Advanced Bioscience, Keio University, Tokyo, Japan
1 Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Fukuoka, Japan
2 Institute of Advanced Bioscience, Keio University, Tokyo, Japan
Effect of the specific gene knockout on the main metabolism in Escherichia coli was reviewed, and the regulation mechanisms were clarified based on different levels of information such as gene expressions, enzyme activities, intracellular metabolite concentrations, and metabolic fluxes together with fermentation data. The effects of the knockout of such genes as pflA , pta , ppc , pykF , adhE , and ldhA on the metabolic changes were analyzed for the case under anaerobic condition. The effects of the knockout of such genes as pgi , zwf , gnd , ppc pck , pyk , and lpdA on the metabolic changes were also analyzed for the case under aerobic condition. The metabolic regulation analysis was made focusing on the roles of transcription factors.
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