Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α 6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α 6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treat ing oocytes with α 6 integrin subunit antibody. The α 6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was sig nificantly decreased when in vitro matured bovine oocytes were pre-incubated with α 6 integrin subunit antibody at concentration 5 and 20 μg/mL, and sperm oocyte binding increased. These studies demonstrated the presence of α 6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.
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