Characterization of purified <i>β</i>-glucosidase produced from <i>Trichoderma viride</i> through bio-processing of orange peel waste

Abstract

In the present study , solid state fermentation was carried out using orange peel waste to produce β -glucosidase from Trichoderma viride . A locally isolated fungal strain T . viride was cultured in the solid state medium of orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity of 515 ± 12.4 U/mL was recorded after 4 th day of incubation at pH 5.5 and 30℃ . Indigenously produced β -glucosidase was subjected to the ammonium sul f ate precipitation and Sephadex-G-100 gel filtration chromatography . I n comparison to the crude extract β -glucosidase was 5.1 - fold purified with specific activity of 758 U/mg. The enzyme was shown to have a relative molecular weight of 6 2 kDa as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified β -glucosidase displayed 6 and 60℃ as an optimum pH and temperature respectively.