Genetic variability of the Saudi Arabian Uromastyx aegyptia microlepis using protein and isoenzymes electrophoreses
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Abstract
Electrophoresis for SDS-proteins and isoenzymes were conducted to investigate the genetic variations within the agamid lizard Uromastyx aegyptia microlepis inhabiting the desert of Saudi Arabia. Samples were collected from four localities: a) Ushayrah near the town of Taif, b) Al Gwaih near Riyadh, c) Nairyah near Dammam and d) Mouileh near Tabok. A range of 7 to 14 protein bands were recorded in the patterns of the studied samples as measured by SDS-polyacrylamide gel electrophoresis. Among them, only one fraction was recorded in all samples as a common protein band. Six arbitrary chosen enzymes were examined by native-polyacrylamide gel electrophoresis. They were α and β esterase (Est), acid phosphatase (Acph), Alcohol dehydrogenase (Adh), Aldehyde oxidase (Ao) and peroxidase (Px). Seventeen heterogeneous alleles have been recorded; seven of them were fixed in all populations and 10 were polymorphic. Nearly all recorded alleles were monomeric in all samples. α-Est2, β-Est2, Acph2 and Px1 were restricted to Tabok samples and were not recorded in other localities. β-Est3, Acph3, Adh1, Adh1and Px2 were not recorded in Taif samples and the latter one was not recorded in the Dammam samples. The similarity coefficient that has been calculated according to the number of sharing bands indicated the clustering of Tabok and Dammam populations together. The constructed tree based on the sharing protein bands and isozyme alleles revealed similar results regarding the kinship of both populations. The present results indicated that the populations of this subspecies exhibits high genetic variability among its populations.
- Wilms, T. and B?hme, W. (2000) A new Uromastyx species from south-eastern Arabia, with comments on the taxonomy of Uromastyx aegyptia (Forsk?l, 1775) (Squamata: Sauria: Agamidae). Herpetology, 13, 133-148.
- Wilms, T., B?hme, W., Wagner, P., Lutzmann, N. and Schmitz, A. (2009a) On the phylogeny and taxonomy of the genus Uromastyx Merrem, 1820 (Reptilia: Squamata: Agamidae: Uromastycinae)—Resurrection of the genus Saara Gray, 1845. Bonner zoologische Beitr?ge, 56, 55-99.
- Wilms, T. and B?hme, W. (2007) Review of the taxonomy of the spiny-tailed lizards of Arabia (Reptilia: Agamidae: Leiolepidinae: Uromastyx). Fauna of Arabia, 23, 435-468.
- Arnold, E.N. (1986) A key and annotated check-list to the lizards and amphibians of Arabia. Fauna of Saudi Arabia, 8, 385-435.
- Joger, U. ( 1991) A molecular phylogeny of agamid lizards. Copeia, 3, 616-622. doi:10.2307/1446389
- Mertens, R. (1956) Amphibien und Reptilien aus SO?Iran 1954. Jahreshefte des Vereins für vaterl?ndische Naturkunde in Württemberg, 111, 90-97.
- Moody, S.M. (1987) A preliminary cladistic study of the lizard genus Uromastyx (Agamidae, sensu lato), with a checklist and diagnostic key to the species. In: van Gelder, J.J., Strijbosch, H. and Bergers, P.J.M. Eds., Proceedings of the 4th Ordinary General Meeting of the Society of European Herpetology, Nijmegen, Holland, 285-288.
- Amer, S.A.M. and Kumazawa, Y. (2005) Mitochondrial DNA sequences of the Afro-Arabian spiny tailed lizards (genus Uromastyx; family Agamidae): phylogenetic analyses and evolution of gene arrangements. Biological Journal of the Linneaen Society, 85, 247-260. doi:10.1111/j.1095-8312.2005.00485.x
- Amer, S.A.M. and Kumazawa, Y. (2009) Molecular affinity of Somali and Egyptian mastigures among the Afro-Arabian Uromastyx. Egyptian Journal of Experimental Biology, Zoology, 5, 1-7.
- Harris, D.J., Vaconcelos, R. and Brito, J.C. (2007) Genetic variation within African spiny-tailed lizards (Agamidae: Uromastyx) estimated using mitochondrial DNA sequences. Amphibia-Reptilia, 28, 1-6. doi:10.1163/156853807779799144
- Wilms, T. and Schmitz, A. (2007) A new polytypic species of the genus Uromastyx Merrem1820 (Reptilia: Squamata: Agamidae: Leiolepidinae) in southwestern Arabia. Zootaxa, 1394, 1-23.
- Al-Seeni, A.E. (2009) Some Biochemical Studies on the Aldehyde Oxidase and Xanthine Oxidase of Agamid Lizard Uromastyx microlepis (Dhubb). World Journal of Chemistry, 4, 45-51.