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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells
Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK
Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK
Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK
- 1 Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK
- 2 Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK
- 3 Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK
Advances in Bioscience and Biotechnology·Volume 04 (2013)·Pages 1013–1019·Published 23 December 2013·DOI10.4236/abb.2013.412135
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Abstract
Three transfection reagents, Lipofectamine ® 2000, TransIT-PRO ® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO ® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.
KeywordsTransient Gene ExpressionTransfection EfficiencyChinese Hamster Ovary CellsmRNA StabilityTemperature Shift
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