Reactive oxygen species—Control and management using amphiphilic biosynthetic hydrogels for cardiac applications

Abstract

The reactive oxygen species (ROS) originated from endogenous and exogenous sources play a dominant role in the initiation and propagation of several diseases. It is therefore an urgent need to explore substances capable of encountering the ROS and resist the damage caused by ROS. The present paper deals with various aspects of generation and implications of ROS in the management of myocardial infarction. The use of biosynthetic amphiphilic biodegradable hydrogels in the control and management of ROS in myocardial infarction was studied using a biosynthetic hydrogel (PA-PEGDA) comprising poly(propylene fumarate)-co-alginate copolymer cross-linked with calcium and polyethylene glycol diacrylate (PEGDA). The effect of ROS on the cell growth was studied using H 2 O 2 as model ROS molecule. The present hydrogel resists the penetration of ROS in the cell which was evident from the live/dead assay, increased intra cellular GSH levels when compared with the H 2 O 2 treated positive and curcumin treated negative control cells. The Comet assay reveals genomic integrity of the cells exposed to the present hydrogel. The hydrogel is a promising injectable material for the management of myocardial infarction and ischemia.