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Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (<i>WT</i>1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry
Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA; Northwestern University Feinberg School of Medicine, Chicago, USA
- 1 Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
- 2 Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
- 3 Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA
- 4 Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA; Northwestern University Feinberg School of Medicine, Chicago, USA
Advances in Bioscience and Biotechnology·Volume 05 (2014)·Pages 155–159·Published 24 January 2014·DOI10.4236/abb.2014.52019
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Abstract
This study was to determine whether GM-CSF induced WT 1 gene expression and to establish an association with markers of proliferation CD71 + CD34 + using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT 1 gene expression and CD71 + CD34 + in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT 1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.
KeywordsJuvenile Myelo-Monocytic LeukemiaWilms TumorNested PCRJMML<i>WT</i>1GM-CSFnPCR
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