<i>Pseudomonas aeruginosa</i> BUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine

Abstract

This study focuses on the isolation and characterization of a novel strain of siderophore producing bacterium, i.e ., Pseudomonas aeruginosa BUP2 ( Pa BUP2) from the rumen of Malabari goat, coupled with qualitative and quantitative analyses of the siderophore produced by it. Pa BUP2 — a facultative anaerobe was tuned to be an aerobe by repeatedly growing in Benjamin flask . The new isolate was grown in a specially designed semi-synthetic medium, designated as BUP medium, and the yellowish-green pigment produced was identified as a typical siderophore by spectrophotometry, Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF). The characteristic orange fluorescence upon UV irradiation on chromatogram and absorption maximum at λ 404 confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further categorized under type 2 by comparing its profile on the IEF gel with that of the representative strains of each PVD types, viz ., Pa O1, Pa ATCC 27853 and Pa 6. Moreover, the type 2 PVD was purified by XAD-4 Amberlite column chromatography and quantified; maximum yield (11.17 mg/ml) was observed on day 4 of incubation ( 37 ° C ). Thus, it was confirmed that the bacterium isolated from the rumen content of Malabari goat is a novel strain of Pa capable of producing large quantity of PVD type 2 in specially designed BUP medium under aerobic condition, and that its clinical and industrial implications remain elusive.