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Genetic diversity among Indian phytopathogenic isolates of Fusarium semitectum Berkeley and Ravenel
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Advances in Bioscience and Biotechnology·Volume 02 (2011)·Pages 142–148·Published 23 May 2011·DOI10.4236/abb.2011.23023
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Abstract
We report total ten isolates of F. semitectum recovered from different hosts. Identity of these isolates was determined by morphological and cultural characteristics and confirmed by RAPD-PCR analysis using forty random primers. Morphologically all the ten isolates showed similarity, but based on RAPD-PCR analysis, these isolates can be categorized in three groups depending upon similarity co-efficient. Genetic similarity coefficients between pair wise isolates varied from 0.00 to 1.95 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation among isolates of F. semitectum.
KeywordsFusariumGenetic DiversityRAPDMacroconidiaUPGMA
- Ingle, A., Karwa, A., Rai, M.K. and Gherbawy, Y. (2009) Fusarium: molecular detection, mycotoxins and biocontrol. In: Gherbawy, Y., Mach, R. and Rai, M. Eds., Current Advances in Molecular Mycology, Science Publishers Inc., Enfield, 85-106.
- Steinkellner, S., Mammerler R. and Vierheilig, H. (2008) Germination of Fusarium oxysporum in root exudates from tomato plants challenged with different Fusarium oxysporum strains. European Journal Plant Pathology, 122, 395-401. doi:10.1007/s10658-008-9306-1
- Godoy, P., et al. (2004) Onychomycosis caused by Fusarium solani and Fusarium oxysporum in S?o Paulo, Brazil. Mycopathologia, 157, 287-290. doi:10.1023/B:MYCO.0000024186.32367.d4
- Mansoory, D., Roozbahany, N.A., Mazinany, H. and Samimagam, A. (2003) Chronic Fusarium infection in an adult patient with undiagnosed chronic granulomatous disease. Clinical Infectious Diseases, 37, 107-108. doi:10.1086/377608
- Nucci, M. and Anaissie, E. (2007) Fusarium infections in immunocompromised patients. Clinical Microbiology Review, 20, 695-704. doi:10.1128/CMR.00014-07
- Sultana, N. and Hanif, N.Q.N. (2009) Mycotoxin contamination in cattle feed and feed ingredients. Pakistan Veterinary Journal, 29, 211-213.
- Girish, G.K. and Goyal, R.K. (1986) Prevention and control of mycotoxins in food grains in India. Bull Grain Technology, 24, 157-177.
- Knight, C., Cutts, D.F. and Colhoun, J. (2008) The role of Fusarium semitectum in causing crown rot of banana. Journal Phytopathology, 89, 170-176. doi:10.1111/j.1439-0434.1977.tb02853.x
- Hawa, M.M., Salleh, B. and Latiffah, Z. (2010) Characterization and intraspecific variation of Fusarium semitectum (Berkeley and Ravenel) associated with red-fleshed dragon fruit (Hylocereus polyrhizus [Weber] Britton and Rose) in Malaysia. African Journal Biotechnology, 9, 273-284.
- Mikunthan, G. and Manjunatha, M. (2006) Fusarium se- mitectum, a potential mycopathogen against thrips and mites in chilli, Capsicum annuum. Communications in Agricultural and Applied Biological Sciences, 71, 449-463.
- Manjunatha, A.M., et al. (2009) Evaluation of fungal pathogen, Fusarium semitectum Berk and Ravenel against tobacco aphid under laboratory and greenhouse conditions. Karnataka Journal of Agriculture Science, 22, 495-498.
- Seifert, K. (1996) Fusarium interactive key. Agriculture and Agri food Canada, 1-30.