Characterization and Antioxidant Properties of OJP2, a Polysaccharide Isolated from <i>Ophiopogon japonicus</i> — Oak Academic Publishing
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Characterization and Antioxidant Properties of OJP2, a Polysaccharide Isolated from <i>Ophiopogon japonicus</i>
Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Institute of Glycobiological Engineering, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
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Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
1 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
2 Institute of Glycobiological Engineering, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
3 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
4 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
5 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
6 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
7 Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou, China
A water-soluble polysaccharide (OJP2) obtained from the roots of Ophiopogon japonicas , was precipitated with 95% ethanol and purified by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography. The characteristics of OJP2 were determined by chemical analysis, high performance gel permeation chromatography (HPGPC), and gas chromatography-mass spectrometry (GC-MS). The results showed that the average molecular weight (Mw) of OJP2 was 35.2 kDa, and five kinds of monosaccharides including rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 0.5:5:4:1:10. Furthermore, the antioxidant activity of OJP2 was evaluated in H 2 O 2 -treated HaCaT cells and glucose-treated LO2 cells. The results show that OJP2 can increase the activity of SOD and NO production, and decrease the level of MDA in these two kinds of injury cells. OJP2 should be explored as a novel and potential natural antioxidant agent for use in functional foods or medicine.
Kozarski, M., Klaus, A., Niksic, M., Jakovljevic, D., Helsper, J.P.F.G. and Van Griensven, L.J.L.D. (2011) Antioxidative and Immunomodulating Activities of Polysaccharide Extracts of the Medicinal Mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chemistry, 129, 1667-1675. http://dx.doi.org/10.1016/j.foodchem.2011.06.029
Wu, X.J. and Hansen, C. (2008) Antioxidant Capacity, Phenol Content, and Polysaccharide Content of Lentinus edodes Grown in Whey Permeate-Based Submerged Culture. Journal of Food Science, 73, 1-8. http://dx.doi.org/10.1111/j.1750-3841.2007.00595.x
Tsai, M., Song, T., Shih, P. and Yen, G. (2007) Antioxidant Properties of Water-Soluble Polysaccharides from Antrodia cinnamomea in Submerged Culture. Food Chemistry, 104, 1115-1122. http://dx.doi.org/10.1016/j.foodchem.2007.01.018
Thetsrimuang, C., Khammuang, S., Chiablaem, K., Srisomsap, C. and Sarnthima, A. (2011) Antioxidant Properties and Cytotoxicity of Crude Polysaccharides from Lentinus polychrous Lév. Food Chemistry, 128, 634-639. http://dx.doi.org/10.1016/j.foodchem.2011.03.077
Wu, X.J. and Hansen, C. (2008) Antioxidant Capacity, Phenol Content, and Polysaccharide Content of Lentinus edodes Grown in Whey Permeate-Based Submerged Culture. Journal of Food Science, 73, 1-8. http://dx.doi.org/10.1111/j.1750-3841.2007.00595.x
Xu, W., Zhang, F., Luo, Y., Ma, L., Kou, X. and Huang, K. (2009) Antioxidant Activity of a Water-Soluble Polysaccharide Purified from Pteridium aquilinum. Carbohydrate Research, 344, 217-222. http://dx.doi.org/10.1016/j.carres.2008.10.021
Shin, K.H., Lim, S.S., Lee, S.H., Lee, Y.S. and Cho, S.Y. (2001) Antioxidant and Immunostimulating Activities of the Fruiting Bodies of Paecilomyces japonica, a New Type of Cordyceps sp. Annals of the New York Academy of Sciences, 928, 261-273. http://dx.doi.org/10.1111/j.1749-6632.2001.tb05655.x
Finkel, T. and Holbrook, N.J. (2000) Oxidants, Oxidative Stress and the Biology of Aging. Nature, 408, 239-247. http://dx.doi.org/10.1038/35041687
Xue, S.X., Chen, X.M., Lu, J.X. and Jin, L.Q. (2009) Protective Effect of Sulfated Achyranthes Bidentata Polysaccharides on Streptozotocin-Induced Oxidative Stress in Rats. Carbohydrate Polymers, 75, 415-419. http://dx.doi.org/10.1016/j.carbpol.2008.08.003
Chen, R.Z., Liu, Z.Q., Zhao, J.M., Chen, R.P., Meng, F.L., Zhang, M. and Ge, W.C. (2011) Antioxidant and Immunobiological Activity of Water-Soluble Polysaccharide Fractions Purified from Acanthopanax senticosu. Food Chemistry, 127, 434-440. http://dx.doi.org/10.1016/j.foodchem.2010.12.143
Chen, X.M., Nie, W.J., Fan, S.R., Zhang, J.F., Wang, Y.X., Lu, J.X. and Jin, L.Q. (2012) A Polysaccharide from Sargassum fusiforme Protects against Immunosuppression in Cyclophosphamide-Treated Mice. Carbohydrate Polymers, 90, 1114-1119. http://dx.doi.org/10.1016/j.carbpol.2012.06.052
Chen, R.Z., Meng, F.L., Liu, Z.Q., Chen, R.P. and Zhang, M. (2010) Antitumor Activities of Different Fractions of Polysaccharide Purified from Ornithogalum caudatum Ait. Carbohydrate Polymers, 80, 845-851. http://dx.doi.org/10.1016/j.carbpol.2009.12.042
Chen, X.M., Nie, W.J., Yu, G.Q., Li, Y.L., Hu, Y.S., Lu, J.X. and Jin, L.Q. (2012) Antitumor and Immunomodulatory Activity of Polysaccharides from Sargassum fusiforme. Food and Chemical Toxicology, 50, 696-700. http://dx.doi.org/10.1016/j.fct.2011.11.015
Zhou, Y.H., Xu, D.S., Feng, Y., Fang, J.N., Xia, H.L. and Liu, J. (2003) Effects on Nutrition Blood Flow of Cardiac Muscle in Mice by Different Extracts in Radix Ophiopogonis. China Journal of Experimental Traditional Medical Formulae, 9, 22-23.
Huang, H.C. and Ni, Z. (2003) Effect of Ophiopogonis on the Auricular Microcirculation in Mice. Shanghai Laboratory Animal Science, 23, 57-58.
Kou, J., Sun, Y., Lin, Y., Cheng, Z., Zheng, W., Yu, B. and Xu, Q. (2005) Anti-Inflammatory Activities of Aqueous Extract from Radix Ophiopogon japonicus and Its Two Constituents. Biological & Pharmaceutical Bulletin, 28, 1234-1238. http://dx.doi.org/10.1248/bpb.28.1234
Zheng, Q., Feng, Y., Xu, D.S., Lin, X. and Chen, Y.Z. (2009) Influence of Sulfation on Anti-Myocardial Ischemic Activity of Ophiopogon japonicus Polysaccharide. Journal of Asian Natural Products Research, 11, 306-321. http://dx.doi.org/10.1080/10286020902727363
Chen, X.M., Tang, J., Xie, W.Y., Wang, J.J., Jin, J., Ren, J., Jin, L.Q. and Lu, J.X. (2013) Protective Effect of the Polysaccharide from Ophiopogon japonicus on Streptozotocin-Induced Diabetic Rats. Carbohydrate Polymers, 94, 378-385. http://dx.doi.org/10.1016/j.carbpol.2013.01.037
Fan, J. and Zhang, X. (2006) Research Progress in Pharmacology of Ophiopogon japonicus Polysaccharides. Chinese Archives of Traditional Chinese Medicine, 24, 626-627.
Chen, X.M., Jin, J., Tang, J., Wang, Z.F., Wang, J.J., Jin, L.Q. and Lu, J.X. (2011) Extraction, Purification, Characterization and Hypoglycemic Activity of a Polysaccharide Isolated from the Root of Ophiopogon japonicus. Carbohydrate Polymers, 83, 749-754. http://dx.doi.org/10.1016/j.carbpol.2010.08.050
Staub, A.M. (1965) Removal of Proteins from Polysaccharides. Methods in Carbohydrate Chemistry, 5, 5-7.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Colorimetric Method for Determination of Sugars and Related Substance. Analytical Chemistry, 28, 350-356. http://dx.doi.org/10.1021/ac60111a017
Needs, P.W. and Selvendran, R.R. (1993) Avoiding Oxidative Degradation during Sodium Hydroxide/Methyl Iodide-Mediated Carbohydrate Methylation in Dimethyl Sulfoxide. Carbohydrate Research, 245, 1-10. http://dx.doi.org/10.1016/0008-6215(93)80055-J
Yagi, K. (1994) Lipid Peroxides and Related Radicals in Clinical Medicine. In: Armstrong, D., Ed., Free Radicals in Diagnostic Medicine, Plenum Press, New York, 1-14. http://dx.doi.org/10.1007/978-1-4615-1833-4_1
Zhu, M.Y., Mo, J.G., He, C.S., Xie, H.P., Ma, N. and Wang, C.J. (2010) Extraction, Characterization of Polysaccharides from Lycium barbarum and Its Effect on Bone Gene Expression in Rats. Carbohydrate Polymers, 80, 672-676. http://dx.doi.org/10.1016/j.carbpol.2009.11.038
Zhao, G.H., Kan, J.Q., Li, Z.X. and Chen, Z.D. (2005) Structural Features and Immunological Activity of a Polysaccharide from Dioscorea opposita Thunb Roots. Carbohydrate Polymers, 61, 125-131. http://dx.doi.org/10.1016/j.carbpol.2005.04.020
Yang, L. and Zhang, L.M. (2009) Chemical Structural and Chain Conformational Characterization of Some Bioactive Polysaccharides Isolated from Natural Sources. Carbohydrate Polymers, 76, 349-361. http://dx.doi.org/10.1016/j.carbpol.2008.12.015
Sweet, D.P., Shapiro, R.H. and Albersheim, P. (1975) Quantitative Analysis by Various g.l.c. Response-Factor Theories for Partially Methylated and Partially Ethylated Alditol Acetates. Carbohydrate Research, 40, 217-225. http://dx.doi.org/10.1016/S0008-6215(00)82604-X
Misthos, P., Katsaragakis, S., Milingos, N., Kakaris, S., Sepsas, E., Athanassiadi, K., Theodorou, D. and Skottis, I. (2005) Postresectional Pulmonary Oxidative Stress in Lung Cancer Patients. The Role of One-Lung Ventilation. European Journal of Cardiothoracic Surgery, 27, 379-383. http://dx.doi.org/10.1016/j.ejcts.2004.12.023
Dadé, M.M., Schinella, G.R., Fioravanti, D.E. and Tournier, H.A. (2011) Antioxidant and Cytotoxic Properties of an Aqueous Extract from the Argentinean Plant Hedeoma multiflorum. Pharmaceutical Biology, 49, 633-639. http://dx.doi.org/10.3109/13880209.2010.526949
Yao, D.C., Shi, W.B., Gou, Y.L., Zhou, X.R., Tak, Y.A. and Zhou, Y.K. (2005) Fatty Acid-Mediated Intracellular Iron Translocation: A Synergistic Mechanism of Oxidative Injury. Free Radical Biology and Medicine, 39, 1385-1398. http://dx.doi.org/10.1016/j.freeradbiomed.2005.07.015
Karthikeyan, K., Bai, B.R. and Devaraj, S.N. (2007) Cardioprotective Effect of Grape Seed Proanthocyanidins on Isoproterenol-Induced Myocardial Injury in Rats. International Journal of Cardiology, 115, 326-333. http://dx.doi.org/10.1016/j.ijcard.2006.03.016
Wattanapitayakul, S.K., Weinstein, D.M., Holycross, B.J. and Bauer, J.A. (2000) Endothelial Dysfunction and Peroxynitrite Formation Are Early Events in Angiotensin-Induced Cardiovascular Disorders. The FASEB Journal, 14, 271-278.
Hamed, S., Brenner, B., Aharon, A., Daoud, D. and Roguin, A. (2009) Nitric Oxide and Superoxide Dismutase Modulate Endothelial Progenitor Cell Function in Type 2 Diabetes Mellitus. Cardiovascular Diabetology, 8, 56. http://dx.doi.org/10.1186/1475-2840-8-56
Mendez, I.I., Chung, Y.H., Jun, H.S. and Yoon, J.W. (2004) Immunoregulatory Role of Nitric Oxide in Kilham Rat Virus-Induced Autoimmune Diabetes in DR-BB Rats. The Journal of Immunology, 173, 1327-1335. http://dx.doi.org/10.4049/jimmunol.173.2.1327