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Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
- 1 National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
- 2 National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
- 3 National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
- 4 National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
- 5 National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Reproductive Immunology, National Research Institute for Health and Family Planning, Beijing, China
Advances in Bioscience and Biotechnology·Volume 06 (2015)·Pages 624–628·Published 7 September 2015·DOI10.4236/abb.2015.69065
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Abstract
The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.
KeywordsGlycopeptideLC-ESIFTICR-MS
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