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Generation of Electricity by Electrogenic Bacteria in a Microbial Fuel Cell Powered by Waste Water
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 1 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 2 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 3 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 4 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 5 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 6 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 7 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 8 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
- 9 Department of Biological Science and Chemistry, University of Nizwa, Nizwa, Oman
Advances in Bioscience and Biotechnology·Volume 07 (2016)·Pages 329–335·Published 30 June 2016·DOI10.4236/abb.2016.77031
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Abstract
The present study aimed at isolation characterization and evaluation of electrogenic bacteria for electricity generation using waste water. In this context, waste water samples were collected from University of Nizwa waste water treatment plant. A total of eight distinct bacterial isolates were isolated from these samples by serial dilution and plating on LB Agar medium. The bacterial isolates were than grown at different temperatures and pH. DNA from bacterial samples was isolated and 16S rRNA gene amplification was carried out. The 16S rRNA gene PCR products were directly sequenced and the resulting sequence was blasted using BLASTn. Based on BLAST results, the bacterial strains were identified. The bacteria were used in different combinations to generate electricity from waste water in microbial fuel cells constructed using plastic bottles. The microbial isolates were found to produce varying levels of currents and their electrogenic potential in waste water was observed to increase with the passage of time.
KeywordsElectricity ProductionElectrogenic BacteriaWaste WaterMicrobial Fuel Cell
- Khanal, S.K. (2008) Anaerobic Biotechnology for Bioenergy Production: Principles and Applications, John Wiley and Sons Inc., Iowa. http://dx.doi.org/10.1002/9780813804545
- Rabaey, K., Lissens, G., Siciliano, S.D. and Verstraete, W. (2003) A Microbial Fuel Cell Capable of Converting Glucose to Electricity at High Rate and Efficiency. Biotechnology Letters, 25, 1531-1535. http://dx.doi.org/10.1023/A:1025484009367
- Liu, H. and Logan B.E. (2004) Electricity Generation Using an Air Cathode Single Chambers Microbial Fuel Cell in the Presence and Absence of a Proton Exchange Membrane. Environmental Science & Technology, 38, 4040-4046. http://dx.doi.org/10.1021/es0499344
- Rabaey, K., Rodríguez, J., Blackall, L.L., Keller, J., Gross, P., Batstone, D., et al. (2007) Microbial Ecology Meets Electrochemistry: Electricity-Driven and Driving Communities. The ISME Journal, 1, 9-18. http://dx.doi.org/10.1038/ismej.2007.4
- Logan, B.E., Hamelers, B., Rozendal, R., Schroder, U., Keller, J., Freguia, S., et al. (2006) Microbial Fuel Cells: Methodology and Technology. Environmental Science & Technology, 40, 5181-5192. http://dx.doi.org/10.1021/es0605016
- Rabaey, K., Boon, N., Siciliano, S.D., Verhaege, M. and Verstraete, W. (2004) Biofuel Cells Select for Microbial Consortia That Self-Mediate Electron Transfer. Applied and Environmental Microbiology, 70, 5373-538. http://dx.doi.org/10.1128/AEM.70.9.5373-5382.2004
- Logan, B.E. (2008) Microbial Fuel Cells. John Wiley & Sons, Inc., New York.
- Lovley, D.R. (2006) Bug juice: Harvesting Electricity with Microorganisms. Nature Reviews Microbiology, 4, 497-508. http://dx.doi.org/10.1038/nrmicro1442
- Richter, H., Lanthier, M., Nevin, K.P. and Lovley, D.R. (2007) Lack of Electricity Production by Pelobacter carbinolicus Indicates That the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes. Applied and Environmental Microbiology, 73, 5347-5353. http://dx.doi.org/10.1128/AEM.00804-07
- Bretschger, O., Obraztsova, A., Sturm, C.A., Chang, I.S., Gorby, Y.A., Reed, S.B., et al. (2007) Current Production and Metal Oxide Reduction by Shewanellaoneidensis MR-1 Wild Type and Mutants. Applied and Environmental Microbiology, 73, 7003-7012. http://dx.doi.org/10.1128/AEM.01087-07
- Allen, R.M. and Bennetto, H.P. (1993) Microbial Fuel Cells: Electricity Production from Carbohydrates. Applied Biochemistry and Biotechnology, 39, 27-40. http://dx.doi.org/10.1007/BF02918975