Effect of antigens on colony forming efficiency of stromal clonogenic cells and expression of cytokine genes in primary cultures of bone marrow and spleen of mice
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Abstract
Presence of microbial cells preparation (HCL – extract of group A streptococcus) in primary cell cultures of bone marrow (BMCC) and spleen (SCC) of CBA mice induced the expression mRNA of proinflammatory cytokines genes: IL-2, IL-6 and IL-8 in BMCC; in SCC cultures mRNA IL-1 β and IL-6 appeared and mRNA IL-4 disappeared. Injection of S. typhimurium antigen complex to mice CBA increased 5 times colony forming efficiency (CFE) and, respectively, content of stromal precursor cells (CFU-F) in femur bone marrow and 9 times in spleen of these animals with maximum at the first day. In both primary BMCC and SCC from immunized animals expression of proinflammatory cytokines genes IL-1 β, IL-6, IL-8 and TNF- α was revealed - in BMCC on 1 - 3 day after immunization, and in SCC – up to 15 days. In CBA mice injected with polyvinylpyrrolidone (PVP) CFE and number of CFU-F in BMCC and SCC increased 1, 9 and 1, 8 times only and expression of genes IL-6, IL-8 and TNF- was observed only on the first day. In СВА/N xid mice there was neither increase CFU-F in the corresponding organs, nor expression of proinflammatory cytokines genes in primary cultures. The data suggest the possibility of positive participation of stromal cells in the development on of immune response in organism. Degree of activation of stromal tissue in immunized mice, apparently, correlates with the degree of immune response, supposing a close relationship between stromal tissue and immune system.
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