Exclusively Brazilian, the Caatinga biome has been thus far a place of very few studies on the Basidiomycetes fungi. Due to its semiarid climate, fungi found in the region are likely to carry lignolytic enzymes which hold biotechnological potential to be used in industrial processes of agro-industrial residue bioconversion. This study performed a response surface statistical planning to optimize the secretion of enzymes such as laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) by Lentinus crinitus . Three variables were under analysis: different concentrations of barley and cassava residue, pH and temperature. MnP enzyme showed the highest enzymatic activity rate (23.5 IU/L). Additionally, MnP had the best results of enzyme secretion for substrate composition of 50% barley and 50% cassava, at pH 7 and temperature at 28°C for a 28-day incubation period. However, further studies are pivotal to test the efficiency in lignin bioconversion by the enzymes synthesized in this work and also to establish their usage pattern on a large scale.
Goes-Neto, A., et al. (2003) Lignicolous Aphyllophoroid Basidiomycota in an Atlantic Forest Fragment in the Semi-Arid Caatinga Region of Brazil. Mycotaxon, 88, 359-364.
Santos, E.R.D. (2010) Diversity of lignolytic Agarycomycetes in the Brazilian Semi-Arid. Ph.D. Thesis, Universidade Federal de Pernambuco, Recife.
Hibbett, D.S., et al. (2007) A Higher-Level Phylogenetic Classification of the Fungi. Mycological Research, 111, 509-547. https://doi.org/10.1016/j.mycres.2007.03.004
Kamida, H.M., et al. (2005) Decolourization of Municipal Effluent and Sludge by Pleurotus sajor-caju and Pleurotus ostreatus. World Journal of Microbiology & Biotechnology, 21, 1363-1369. https://doi.org/10.1007/s11274-005-5143-y
Eriksson, K.E.L. (1990) Biotechnology in the Pulp and Paper Industry. Wood Science and Technology, 24, 79-101. https://doi.org/10.1007/BF00225309
Hofrichter, M., et al. (2010) New and Classic Families of Secreted Fungal Heme Peroxidases. Applied Microbiology and Biotechnology, 87, 871-897. https://doi.org/10.1007/s00253-010-2633-0
Käärik, A. (1965) The Identification on the Mycelia of Wood Decay Fungi by Their Oxidation Reaction with Phenolic Compounds. Studia Florestalia Suecia, 31, 1-80.
Tien, M. and Kirk, T.K. (1984) Lignin-Degrading Enzyme from Phanerochaete chrysosporium: Purification, Characterization and Catalytic Properties of a Unique H2O2 Requiring Oxygenase. Proceeding of the National Academy of Science, 81, 2280-2284. https://doi.org/10.1073/pnas.81.8.2280
Kuwahara, M., et al. (1984) Separation and Characterization of Two Extracellular H2O2 Dependent Oxidases from Lignolytic Cultures of Phanerochaete chrysosporium. FEBS Letters, 169, 247-250. https://doi.org/10.1016/0014-5793(84)80327-0
Morgenstern, I., et al. (2008) Molecular Evolution and Diversity of Lignin Degrading Heme Peroxidases in the Agaricomycetes. Journal of Molecular Evolution, 66, 243-257. https://doi.org/10.1007/s00239-008-9079-3
Xu, H., et al. (2017) Expression and Characteristics of Manganese Peroxidase from Ganoderma Lucidum in Pichia pastoris and Its Application in the Degradation of Four Dyes and Phenol. BMC Biotechnology, 17, 1-12. https://doi.org/10.1186/s12896-017-0338-5
Kadimaliev, D.A., et al. (2008) Increased Secretion of Lignolytic Enzymes by the Lentinus crinitus Fungus after Addition of Butanol and Toluene in Submerged Cultivation. Applied Biochemistry and Microbiology, 44, 528-534. https://doi.org/10.1134/S000368380805013X
Elisashvili, V. and KACHLISHVILI, E. (2009) Physiological Regulation of Laccase and Manganese Peroxidase Production by White-Rot Basidiomycetes. Journal of Biotechnology, 144, 37-42. https://doi.org/10.1016/j.jbiotec.2009.06.020
Hadibarata, T., et al. (2009) Biodegradation of Chrysene, an Aromatic Hydrocarbon by Polyporus sp. S133 in Liquid Medium. Journal of Hazardous Materials, 164, 911-917. https://doi.org/10.1016/j.jhazmat.2008.08.081
Covino, S., et al. (2010) In Vivo and in Vitro Polycyclic Aromatic Hydrocarbons Degradation by Lentinus (Panus) crinitus CBS 577.79. Bioresource Technology, 101, 3004-3012. https://doi.org/10.1016/j.biortech.2009.12.020
Elisashvili, V., et al. (2010) Effect of Aromatic Compounds on the Production of Laccase and Manganese Peroxidase by White-Rot Basidiomycetes. The Journal of Industrial Microbiology and Biotechnology, 37, 1091-1096. https://doi.org/10.1007/s10295-010-0757-y
Alexandrino, A.M., et al. (2007) Reutilization of Orange Waste for Production of Lignocellulolytic Enzymes by Pleurotus ostreatus (Jack: Fr). Ciência e Tecnologia de Alimentos, 27, 364-368. https://doi.org/10.1590/S0101-20612007000200026
Menezes, C.R., et al. (2009) Sugarcane Bagasse: Source for the Production of Ligninocelullolytic Enzymes. Estudos Tecnológicos, 5, 68-78. https://doi.org/10.4013/ete.2009.51.05
Bilal, M., et al. (2017) Biotransformation of Lignocellulosic Materials into Value-Added Products—A Review. International Journal of Biological Macromolecules, 98, 447-458. https://doi.org/10.1016/j.ijbiomac.2017.01.133
Fleuri, L.F. and Sato, H.H. (2008) Study of Different Parameters in the Production of Lytic Enzymes. Food Science and Technology, 28, 299-310. https://doi.org/10.1590/S0101-20612008000200006
Aanchal, et al. (2016) Response Surface Methodology for Optimization of Microbial Cellulase Production. Romanian Biotechnological Letters, 21, 11832-11841.
Morshedi, A. and Akbarian, M. (2014) Application of Response Surface Methodology: Design of Experiments and Optimization: A Mini Review. Indian Journal of Fundamental and Applied Life Sciences, 4, 2434-2439.
Oss, O.T. and Oeric, O.N. (1986) Psilocybin, Magic Mushroom Growers Guide. Lux Natura, Quick American Archives, San Francisco.
Góes-Neto, A., et al. (2005) DNA Extraction from Frozen Field Collected and Dehydrated Herbarium Fungal Basidiomata: Perform of SDS and CTAB Based Methods. Biotemas, 18, 19-32.
Staden, R., et al. (1998) The Staden Package. Methods in Molecular Biology, 132, 115-130.
Thompson, J.D., et al. (1997) The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 25, 4876-4882. https://doi.org/10.1093/nar/25.24.4876
Szklarz, G., et al. (1984) Production of Phenoloxidases and Peroxidases by Wood-Rotting Fungi. Mycologia, 81, 234-240. https://doi.org/10.2307/3759705
Schmidt, O. and Moreth, U. (2003) Data Bank of rDNA-ITS Sequences from Building-Rot Fungi for Their Identification. Wood Science of Technology, 37, 161-163. https://doi.org/10.1007/s00226-003-0162-z
Badotti, K., et al. (2017) Effectiveness of ITS and Sub-Regions as DNA Barcode Markers for the Identification of Basidiomycota (Fungi). BMC Microbiology, 17-42.
Manpreet, S., et al. (2005) Influence of Process Parameters on the Production of Metabolites in Solid-State Fermentation. Malalaysian Journal of Microbiology, 1, 1-9.
Ellouze, M., et al. (2008) Detoxification of Tunisian Landfill Leachates by Selected Fungi. Journal of Hazardous Materials, 150, 642-648. https://doi.org/10.1016/j.jhazmat.2007.05.013
Chang, B.-V. and Chang, Y.-M., (2014) Biodegradation of Toxic Chemicals by Pleurotus eryngii in Submerged Fermentation and Solid-State Fermentation. Journal of Microbiology, Immunology and Infection, In Press, 1-7.
Gomes, E., et al. (2009) Ligninases Production by Basidiomycetes Strains on Lignocellulosic Agricultural Residues and Their Application in the Decolorization of Synthetic Dyes. Brazilian Journal of Microbiology, 40, 31-39. https://doi.org/10.1590/S1517-83822009000100005
Dinis, M.J., et al. (2009) Modification of Wheat Straw Lignin by Solid State Fermentation with White-Rot Fungi. Bioresource Technology, 100, 4829-4835. https://doi.org/10.1016/j.biortech.2009.04.036
Thiribhuvanamala, G., et al. (2017) Induction of Lignolytic Enzyme Activities in Different Agro Residues by the White Rot Fungi, Pleurotus Sajar-Caju. International Journal of Chemical Studies, 5, 89-94.
Mishra, V., et al. (2017) Enhancement in Multiple Lignolytic Enzymes Production for Optimized Lignin Degradation and Selectivity in Fungal Pretreatment of Sweet Sorghum Bagasse. Bioresource Technology, 236, 49-59. https://doi.org/10.1016/j.biortech.2017.03.148
Bilal, M. and Asgher, M. (2016) Biodegration of Agrowaste by Lingocellulolytic Activity of Oyster Mushroom, Pleurotus Sapidus. Journal of the National Science Foundation of Sri Lanka, 44, 399-407. https://doi.org/10.4038/jnsfsr.v44i4.8022
Irbe, I., et al. (2014) Lignocellulolytic Activity of Coniophora puteana and Trametes versicolor in Fermentation of Wheat Bran and Decay of Hydrothermally Modified Hardwoods. International Biodeterioration & Biodegradation, 86, 71-78. https://doi.org/10.1016/j.ibiod.2013.06.027
Niebisch, C.H. (2009) Biodegradation of Ramazol Blye Dye by Lentinus crinitus, Lepista sordida e Hydnopolyporus fimbriatus. Master’s Thesis, Universidade Federal do Paraná, Curitiba.