Effects of Pyridoxine on Selected Appetite Regulating Peptides mRNA Expression in Hypothalamic PVN/ARC Nuclei and Gastrointestinal Tract Tissues — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Effects of Pyridoxine on Selected Appetite Regulating Peptides mRNA Expression in Hypothalamic PVN/ARC Nuclei and Gastrointestinal Tract Tissues
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Sciences, Jinan, China
,
Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
,
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
1 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
2 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
3 Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Sciences, Jinan, China
4 Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, China
5 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
6 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
7 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
8 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
9 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
10 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Technology, Shandong Agricultural University, Taian, China
An experiment was conducted to investigate the effect of dietary pyridoxine on the gene expression of appetite-regulating peptides in the hypothalamus and gastrointestinal tract of rabbits. Thirty-two rabbits were randomly divided into 2 treatments for 8 weeks (16 replicates/group and 1 rabbit/replicate). The treatments were fed a basal diet (control, measured pyridoxine content is 4.51 mg/kg) and the basal diet with a pyridoxine supplementation at 10 mg/kg (pyridoxine, measured pyridoxine content is 14.64 mg/kg). The results showed that dietary pyridoxine did not significantly alter the mRNA levels of neuropeptide Y, agouti related peptide, pro-opiomelanocortin and cocaine, amphetamine regulated transcript, peptide YY and cholecystokinin in arcuate nucleus, peptide YY in jejunum and ileum, and cholecystokinin in duodenum, jejunum and ileum ( P > 0.05). Compared with the control, the mRNA levels of corticotropin-releasing hormone and melanocortin 4 receptor in paraventricular nuclei and peptide YY in duodenum were significantly decreased after pyridoxine treatment ( P < 0.05). In conclusion, the appetite genes of melanocortin 4 receptor and corticotropin-releasing hormone in paraventricular nuclei and peptide YY in duodenum are involved in the pyridoxine-caused hyperphagia.
KeywordsPyridoxineBrain-Gut PeptidesAppetite Control
Elias, C.F., Lee, C., Kelly, J., et al. (1998) Leptin Activates Hypothalamic CART Neurons Projecting to the Spinal Cord. Neuron, 21, 1375-1385. https://doi.org/10.1016/S0896-6273(00)80656-X
Kristensen, P., Judge, M.E., Thim, L., et al. (1998) Hypothalamic CART Is a New Anorectic Peptide Regulated by Leptin. Nature, 393, 72-76. https://doi.org/10.1038/29993
Broberger, C., Johansen, J., Johansson, C., Schalling, M. and Hokfelt, T. (1998) The Neuropeptide Y/Agouti Gene-Related Protein (AGRP) Brain Circuitry in Normal, Anorectic, and Monosodium Glutamate-Treated Mice. PNAS, 95, 15043-15048. https://doi.org/10.1073/pnas.95.25.15043
Boswell, T. (2005) Regulation of Energy Balance in Birds by the Neuroendocrine Hypothalamus. Journal Poultry Science, 42, 161-168. https://doi.org/10.2141/jpsa.42.161
Vila G., Hopfgartner, J., Grimm, G., Baumgartner-Parzer, S.M., et al. (2015) Lactation and Appetite-Regulating Hormones: Increased Maternal Plasma Peptide YY Concentrations 3-6 Months Postpartum. British Journal of Nutrition, 24, 1-6. https://doi.org/10.1017/S0007114515002536
Duraisamy, A.J., Bayen, S., et al. (2015) Changes in Ghrelin, CCK, GLP-1, and Peroxisome Proliferator-Activated Receptors in a Hypoxia-Induced Anorexia Rat Model. Endokrynologia Polska, 66, 334-341. https://doi.org/10.5603/EP.2015.0043
Calderón-Guzmán, D., Hernández-Islas, J.L., Espitia-Vázquez, I., et al. (2004) Pyridoxine, Regardless of Serotonin Levels, Increases Production of 5-Hydroxytryptophan in Rat Brain. Archives of Medical Research, 35, 271-274. https://doi.org/10.1016/j.arcmed.2004.03.003
Kratzer, F.H., Bird, F.H., Asmundson, V.S. and Lepkovsky, S. (1947) The Comparative Pyridoxine Requirements of Chicks and Turkey Poults. Poultry Science, 26, 453-456. https://doi.org/10.3382/ps.0260453
Lehrer, W.P., Wiese, A.C., Moore, P.R. and Ensminger, M.E. (1951) Pyridoxine Deficiency in Baby Pigs. Journal of Animal Science, 10, 65-72. https://doi.org/10.2527/jas1951.10165x
Akhtar, M.S., Pal, A.K., Sahu, N.P., Alexander, C. and Meena, D.K. (2012) Effects of Dietary Pyridoxine on Growth and Physiological Responses of Labeo Rohita Fingerlings Reared in High Water Temperature. Israeli Journal of Aquaculture-Bamidgeh, 64, 1-6.
Liu, G., Zhao, N., et al. (2015) Effects of Dietary Vitamin B6 Supplemental Level on Growth Performance and Vitamin B6 Metabolism of Growing Rex Rabbits. Chinese Journal of Animal Nutrition, 27, 2292-2299.
Rogers, K.S. and Mohan, C. (1994) Vitamin B6 Metabolism and Diabetes. Biochemical Medicine and Metabolic Biology, 52, 10-17. https://doi.org/10.1006/bmmb.1994.1027
De Blas, J.C. and Mateos, G.G. (1998) Feed Formulation. In: de Blas, C. and Wiseman, J., Eds., The Nutrition of the Rbbit, Commonwealth Agricultural Bureau, Wallingfold.
Lei, L., Hepeng, L., Xianlei, L., et al. (2013) Effects of Acute Heat Stress on Gene Expression of Brain-Gut Neuropeptides in Broiler Chickens. Journal of Animal Science, 91, 5194-5201. https://doi.org/10.2527/jas.2013-6538
Prior, L.J., Eikelis, N., Armitage, J.A., et al. (2010) Exposure to a High-Fat Diet Alters Leptin Sensitivity and Elevates Renal Sympathetic Nerve Activity and Arterial Pressure in Rabbits. Hypertension, 55, 862-868. https://doi.org/10.1161/HYPERTENSIONAHA.109.141119
Mano-Otagiri, A., Nemoto, T., Sekino, A., et al. (2006) Growth Hormone-Releasing Hormone (GHRH) Neurons in the Arcuate Nucleus (Arc) of the Hypothalamus Are Decreased in Transgenic Rats Whose Expression of Ghrelin Receptor Is Attenuated: Evidence That Ghrelin Receptor Is Involved in the Up-Regulation of GHRH Expression in the Arc. Endocrinology, 147, 4093-4103. https://doi.org/10.1210/en.2005-1619
Freeman, T.C., Bentsen, B.S., Thwaites, D.T. and Simmons, N.L. (1995) H+/ Di-tripeptide Transporter (PepT1) Expression in the Rabbit Intestine. Pflugers Archiv-European Journal of Physiology, 430, 394-400. https://doi.org/10.1007/BF00373915
Liu, L., Song, Z., Jiao, H. and Lin, H. (2014) Glucocorticoids Increase NPY Gene Expression via Hypothalamic AMPK Signaling in Broiler Chicks. Endocrinology, 155, 2190-2198. https://doi.org/10.1210/en.2013-1632
Wang, X.J., Xu, S.H., Liu, L., et al. (2017) Dietary Fat Alters the Response of Hypothalamic Neuropeptide Y to Subsequent Energy Intake in Broiler Chickens. Journal of Experimental Biology, 220, 607-614. https://doi.org/10.1242/jeb.143792
Sanchez-Hernandez, D., Poon, A.N., Kubant, R., et al. (2015) A Gestational Diet High in Fat-Soluble Vitamins Alters Expression of Genes in Brain Pathways and Reduces Sucrose Preference, But Not Food Intake, in Wistar Male Rat Offspring. Applied Physiology Nutrition and Metabolism, 40, 424-431. https://doi.org/10.1139/apnm-2014-0480
Harrold, J.A., Widdowson, P.S. and Williams, G. (1999) Altered Energy Balance Causes Selective Changes in Melanocortin-4 (MC4-R), But Not Melanocortin-3 (MC3-R), Receptors in Specific Hypothalamic Regions: Further Evidence That Activation of MC4-R Is a Physiological Inhibitor of Feeding. Diabetes, 48, 267-271. https://doi.org/10.2337/diabetes.48.2.267
Fan, W., Boston, B.A., Kesterson, R.A., Hruby, V.J. and Cone, R.D. (1997) Role of Melanocortinergic Neurons in Feeding and the Agouti Obesity Syndrome. Nature, 385, 165-168. https://doi.org/10.1038/385165a0
Olszewski, P.K., Bomberg, E.M., Grace, M.K. and Levine, A.S. (2007) Alpha-Melanocyte Stimulating Hormone and Ghrelin: Central Interaction in Feeding Control. Peptides, 28, 2084-2089. https://doi.org/10.1016/j.peptides.2007.07.017
Garg, G., Kumar, J., McGuigan, F.E., et al. (2014) Variation in the MC4R Gene Is Associated with Bone Phenotypes in Elderly Swedish Women. PLoS One, 9, e88565. https://doi.org/10.1371/journal.pone.0088565
Heinrichs, S.C. and Richard, D. (1999) The Role of Corticotropin-Releasing Factor and Urocortin in the Modulation of Ingestive Behavior. Neuropeptides, 33, 350-359. https://doi.org/10.1054/npep.1999.0047
Lu, X.Y., Barsh, G.S., Akil, H. and Watson, S.J. (2003) Interaction between ª-Melanocyte-Stimulating Hormone and Corticotropin-Releasing Hormone in the Regulation of Feeding and Hypothalamo-Pituitary-Adrenal Responses. Journal of Neuroscience, 23, 7863-7872.
Chaudhri, O., Small, C. and Bloom, S. (2006) Gastrointestinal Hormones Regulating Appetite. Philos. Philosophical Transactions of the Royal Society of London Series B—Biological Sciences, 361, 1187-1209. https://doi.org/10.1098/rstb.2006.1856
Coll, A.P., Farooqi, I.S. and O’Rahilly, S. (2007) The Hormonal Control of Food Intake. Cell, 129, 251-262. https://doi.org/10.1016/j.cell.2007.04.001
Rushakoff, R.A., Goldfine, I.D., Beccaria, L.J., et al. (1993) Reduced Postprandial Cholecystokinin (CCK) Secretion in Patients with Noninsulin-Dependent Diabetes Mellitus: Evidence for a Role for CCK in Regulating Postprandial Hyperglycemia. Journal of Clinical Endocrinology and Metabolism, 76, 489-493.
Adrian, T.E., Ferri, G.L., Bacarese-Hamilton, A.J., et al. (1985) Human Distribution and Release of a Putative New Gut Hormone, Peptide YY. Gastroenterology, 89, 1070-1077. https://doi.org/10.1016/0016-5085(85)90211-2
Ballantyne, G.H. (2006) Peptide YY(1-36) and Peptide YY(3-36): Part I. Distribution, Release and Actions. Obesity Surgery, 16, 651-658. https://doi.org/10.1381/096089206776944959
Choi, M., Ozeki, J., Hashizume, M., et al. (2012) Vitamin D Receptor Activation Induces Peptide YY Transcription in Pancreatic Islets. Endocrinology, 153, 5188-5199. https://doi.org/10.1210/en.2012-1396