The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i> — Oak Academic Publishing
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The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i>
Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
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Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
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Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
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Laboratory of Genetic and Molecular Pathology (LGPM), Medical School, Hassan II University, Casablanca, Morocco
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Institute of Biochemistry Vegetal and Photosynthesis (IBVF-CSIC University of Seville), Seville, Spain
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Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
1 Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
2 Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
3 Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
4 Laboratory of Genetic and Molecular Pathology (LGPM), Medical School, Hassan II University, Casablanca, Morocco
5 Institute of Biochemistry Vegetal and Photosynthesis (IBVF-CSIC University of Seville), Seville, Spain
6 Laboratory of Physiopathology, Genetics Molecular and Biotechnology (PGMB), Faculty of Sciences Ain Chock, Research Center: Health & Biotechnology, University Hassan II of Casablanca, Casablanca, Morocco
<i>Staphylococcus aureus</i> is one of the main pathogenic agents responsible for nosocomial and community-acquired bacterial infections. The pathogenicity of this Gram-positive bacterium is ensured by its different adhesion factors. Collagen and the extracellular glycoprotein adhesin are among the <i> Staphylococcus</i> most important virulence factors. It has been shown that most of the <i> S. aureus</i> strains carry the <i> ica</i> operon, responsible for biofilm production. However, the coexpression of the <i> icaA</i> and the <i> icaD</i> genes is necessary for complete biofilm synthesis. The aim of our study was to study a collection of 15 clinical strains of <i> S. aureus</i> from different sources for the presence of <i> can</i> and <i> icaD</i> genes coding intercellular adhesion proteins. We also intended to estimate the strains’ ability to form biofilms by the red Cong method and to test the adhesion ability of <i> S. aureus</i> to the ciliated protist <i> Tetrahymena pyriformis</i> , which we used as a novel cellular adhesion model. Finally, we checked the adhesion’s inhibition capacity of some plants extracts. The molecular detection of adhesion genes revealed that 80% of strains are cna positive, and 73% are <i> icaD</i> positive. Qualitative biofilm production of <i> S. aureus</i> revealed that 66.6% of strains were slime producers. The adhesion test revealed that 20% of strains are strongly adhering to <i> T. pyriformis</i> and that the <i> Clematis cirrhosa</i> extract has an anti-adhering effect of <i> S. aureus</i> to the ciliate <i> T. pyriformis</i> .
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