Molecular Cloning and Phylogenetic Analysis of a Chitin Deacetylase Isolated from the Epidermis of the Red Snow Crab <i>Chionoecetes japonicas</i> — Oak Academic Publishing
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Molecular Cloning and Phylogenetic Analysis of a Chitin Deacetylase Isolated from the Epidermis of the Red Snow Crab <i>Chionoecetes japonicas</i>
Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
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Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
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Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
1 Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
2 Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
3 Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan
Chitin deacetylase (CDA; EC 3. 5. 1. 41) catalyzes the deacetylation of chitin. In this study, we successfully cloned and sequenced a chitin deacetylase gene from the red snow crab <i> Chionoecetes japonicas </i> . By using reverse transcription-polymerase chain reaction (RT-PCR) and 5 ' and 3 ' rapid amplification of cDNA ends, we obtained a 2141-bp amplicon containing a chitin deacetylase gene ( <i> CjCDA </i> ) from the epidermis of <i> C. japonicas </i> . The amplicon contains a 1575-bp open reading frame that is predicted to encode a 525-amino acid protein. The structure predicted from the deduced amino acid sequence in cluded an N-terminal signal peptide, chitin-binding domain (CBD ), low-density lipoprotein receptor class A domain (LDL-A), and catalytic domain. Comparative analysis of the deduced amino acid sequence of <i> CjCDA </i> revealed the highest homology (74%) to gastrolith protein 59 of <i> Cherax quadricarinatus </i> . We used RT-PCR to evaluate the expression of <i> CjCDA </i> in various tissues of <i> C. japonicas </i> , and we observed that <i> CjCDA </i> was expressed only in the epidermis. A phylogenetic analysis, using the amino acid sequences of CjCDA and other known chitin deacetylases, showed that CjCDA belonged to a group of crustacean chitin deacetylases. To our knowledge, this is the first study reporting the cDNA cloning of a chitin deacetylase from a crab.
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