Mercury Distribution in Tobacco (<i>Nicotiana tabacum</i>) Cell
- 1 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
- 2 School of Forestry, Henan University of Science and Technology, Luoyang, China
- 3 The Key Lab of Hainan Banana Genetics and Breeding, Haikou, China
- 4 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
- 5 School of Forestry, Henan University of Science and Technology, Luoyang, China
- 6 School of Forestry, Henan University of Science and Technology, Luoyang, China
- 7 School of Forestry, Henan University of Science and Technology, Luoyang, China
- 8 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
Abstract
Problem : Removing mercury from polluted soil using transgenic plants is an ideal method. However, where mercury was stored in plant cell is not clear until now. Methods : Differential centrifugation and laser scanning confocal microscopy were used in this study. Results and findings : Results showed that after mercury was absorbed by tobacco plants, most of the mercury accumulated in roots. Mercury content in root was significantly higher than that in shoot. In the seedlings cultured in MS liquid medium containing 5 μmol/L mercuric chloride, the mercury content in cell wall was 6.6 μg/g. The mercury content in cell membrane fraction was 2.1 μg/g. The mercury content in supernatant fraction was 35.1 μg/g. Most of the mercury accumulated in roots located in liquid fraction, about 15% of the mercury was attached by cell wall. Only a small part assembled in cell membrane. Most of the mercury in liquid fraction located in vacuole. This suggested that after the mercury was accumulated in plant root, most of the mercury was transferred into vacuole. There was no important cellular organ in vacuole. The toxicity of mercury in vacuole will be much lower than that in cell membrane or cellular organs. Recommendation : These results suggested that much work should be focused on how to transfer the mercury in vacuole into the above-ground tissues of the plants in the future.
- Gong, Y., Liu, Y., Xiong, Z., Kaback, D. and Zhao, D. (2012) Immobilization of Mercury in field Soil and Sediment Using Carboxymethyl Cellulose Stabilized Iron Sulfide Nanoparticles. Nanotechnology, 23, Article ID: 294007. https://doi.org/10.1088/0957-4484/23/29/294007
- Park, C.H., Eom, Y., Lee, L.J. and Lee, T.G. (2013) Simple and Accessible Analytical Methods for the Determination of Mercury in Soil and Coal Samples. Chemosphere, 93, 9-13. https://doi.org/10.1016/j.chemosphere.2013.04.044
- Reis, A.T., Coelho, J.P., Rodrigues, S.M., Rocha, R., Davidson, C.M., Duarte, A.C. and Pereira, E. (2012) Development and Validation of a Simple Thermo-Desorption Technique for Mercury Speciation in Soils and Sediments. Talanta, 99, 363-368. https://doi.org/10.1016/j.talanta.2012.05.065
- Wang, J., Feng, X., Anderson, C.W.N., Wang, H., Zheng, L. and Hu, T. (2012) Implications of Mercury Speciation in Thiosulfate Treated Plants. Environmental Science and Technology, 46, 5361-5368. https://doi.org/10.1021/es204331a
- Jan, A.T., Murtaza, J.I., Ali, A., Mohd, Q. and Haq, R. (2009) Mercury Pollution: An Emerging Problem and Potential Bacterial Remediation Strategies. World Journal of Microbiology and Biotechnology, 25, 1529-1537. https://doi.org/10.1007/s11274-009-0050-2
- Wang, X. and Zhou, Q. (2002) Soil Mercury Pollution and Remediation Technique. Chinese Journal of Ecology, 21, 43-46. (In Chinese)
- Wu, G.H. and Cao, S.S. (2010) Mercury and Cadmium Contamination of Irrigation Water, Sediment, Soil and Shallow Groundwater in a Waste Water-Irrigated Field in Tianjin, China. Bulletin of Environmental Contamination and Toxicology, 84, 336-341. https://doi.org/10.1007/s00128-010-9939-6
- Yang, L., Liu, C., Wang, X., Pei, L., Zhang, Y., Hou, H., Jiang, J. and Song, C. (2010) Soil Contamination Assessment of Main Cities in Henan Province and Its Protection Measures. South-to-North Water Transfers and Water Science and Technology, 8, 94-97. (In Chinese)
- Fang, F.M. and Wang, Q.C. (2000) A Review on the Studies on Mercury Pollution of Soil. Soil and Environmental Sciences, 9, 326-329. (In Chinese)
- Sierra, C., Menendez-Aguado, J.M., Afif, E., Carrero, M. and Gallego, J.R. (2011) Feasibility Study on the Use of Soil Washing to Remediate the As-Hg Contamination at an Ancient Mining and Metallurgy Area. Journal of Hazardous Materials, 196, 93-100. https://doi.org/10.1016/j.jhazmat.2011.08.080
- Zhang, Q., Xu, D.P., Dong, Z.Q., Zhang, X.Y., Li, F.S. and Gu, Q.B. (2012) Effects of Temperature on Mercury Fractions in Contaminated Soil during the Thermal Desorption Process. Research of Environmental Sciences, 25, 870-874. (In Chinese)