Evaluation of Drought Stress-Inducible W<i>si</i>18 Promoter in <i>Brachypodium distachyon</i> — Oak Academic Publishing
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Evaluation of Drought Stress-Inducible W<i>si</i>18 Promoter in <i>Brachypodium distachyon</i>
London Research and Development Centre, Agriculture and Agri-Food Canada, London, Canada
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State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
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Department of Biology, University of Western Ontario, London, Canada
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Department of Biology, Carleton University, Ottawa, Canada
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London Research and Development Centre, Agriculture and Agri-Food Canada, London, Canada
1 London Research and Development Centre, Agriculture and Agri-Food Canada, London, Canada
2 State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
3 Department of Biology, University of Western Ontario, London, Canada
4 Department of Biology, Carleton University, Ottawa, Canada
5 London Research and Development Centre, Agriculture and Agri-Food Canada, London, Canada
The rice Wsi 18 promoter confers drought-inducible gene expression. This property makes it a useful candidate to drive relevant genes for developing drought resistant trait s for different monocot crops. In this study, we showed that the Bradi 2 G 47700 gene, the closest homologue to rice Wsi 18, was upregulated in Brachypodium distachyon plants exposed to ABA and mannitol. Wsi 18: uidA transgenic B. distachyon plants were produced and then subjected to ABA or mannitol treatment. The expression of uidA in three transgenic lines (line 10, 18 and 37) was significantly upregulated in plants exposed to ABA (fold increases of 5.61 ± 0.98, 2.88 ± 0.75 and 9.13 ± 1.96, respectively) compared to the same transgenic plant lines without treatment. The expression of uidA in two transgenic lines (lines 18 and 37) also showed upregulation when treated with mannitol (fold increases of 4.43 ± 1.07 and 8.47 ± 2.90, respectively) compared to the same transgenic plant lines without mannitol treatment. Moreover, GUS histochemical assay showed increased Wsi 18 promoter activity in the leaves and stems of transgenic lines upon treatment with ABA or mannitol. This is the first report of the drought inducible rice Wsi 18 promoter being active in B. distachyon which is a model plant for molecular biology research of various monocot plants. Taken together, the results indicate that the Wsi 18 promoter and its homologue may be explored as a useful tool for drought stress-inducible gene expression in different monocot crops.
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