This study aimed determine the activity of aqueous and ethanolic extracts of Origanum vulgare against some viruses of veterinary importance (bovine viral diarrhea virus (BVDV), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV), canine distemper virus (CDV), canine adenovirus (CAV), and canine cororavirus (CCoV) by evaluating the possibility of inhibition of viral particles production. The aqueous extract from 1600 μg/mL did not show cytotoxicity for all cellular lineages evaluated, Madin Darby bovine kidney cells (MDBK), Rabbit kidney cells (RK 13), Madin Darby canine kidney cells (MDCK) and Crandell feline kidney cells (CRFK), and the ethanolic extract of Origanum vulgare was not toxic at 600 μg/mL. The addition of aqueous extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 10 5.42 infecting dose for cellular culture at 50% (TCID 50 ) to 10 2.09 TCID 50 /100 μL while in the presence of the ethanolic extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 10 5.42 TCID 50 to 10 0.79 TCID 50 /100 μL. To CDV the addition of aqueous extract resulted in a reduction from 10 2.00 TCID 50 to 10 0.00 TCID 50 /100 μL while in the presence of the ethanolic extract titers were reduced from 10 2.00 TCID 50 to 10 1.50 TCID 50 /100 μL. No significant differences in titers regarding the others analyzed viruses were detected. With respect to chemical analysis of the extracts of Origanum vulgare , were identified in the ethanol extract phenolics rosmarinic acid, caffeic acid, carnosol, p-coumaric acid, carnosic acid, luteolin, apigenin, kaempferol and quercetin. In aqueous extracts of Origanum vulgare were detected rosmarinic acid, p-coumaric acid carnosic acid, luteolin, apigenin, kaempferol and quercetin. The data obtained stimulate other biological assays in order to determine which compounds are responsible for the antiviral activity as well as which are the mechanisms involved. The results presented and the considerations we were able to draw from them allowed us to conclude that the ethanolic extract of Origanum vulgare demonstrated lower cell viability than the aqueous extract and has significant antiviral activity against EAV and the both aqueous and ethanolic extracts have antiviral action against CDV.
KeywordsVirus<i>Origanum vulgare</i>
Meyer, G., Deplanche, M., Roux, D., Moulignie, M., Picard-Hagen, N., Lyazrhi, F., Raboisson, D., Mathevet, P. and Schelcher, F. (2012) Fetal Protection against Bovine Viral Diarrhoea Type 1 Virus Infection after One Administration of a Live-Attenuated Vaccine. The Veterinary Journal, 192, 242-245. https://doi.org/10.1016/j.tvjl.2011.05.011
Luo, Y., Yuan, Y., Ankenbauer, R.G., Nelson, L.D., Witte, S.B., Jackson, J.A. and Welch, S.W. (2012) Construction of Chimeric Bovine Viral Diarrhea Viruses Containing Glycoprotein E rns of Heterologous Pestiviruses and Evaluation of the Chimeras as Potential Marker Vaccines against BVDV. Vaccine, 30, 3843-3848. https://doi.org/10.1016/j.vaccine.2012.04.016
Lima, M. and Osorio, F.A. (2007) Arteriviridae. In: Flores, E.F., Eds., Virologia Veterinária, Editora UFSM, Santa Maria/RS, 641-653.
Yuan, C.Z. and Dwight. C.H. (2003) Microbiologia Veterinária Editora: Guanabara.
Muller, V., Chavez, J.H., Reginatto, F.H., Zucolotto, S.M., Niero, R., Navarro, D., Yunes, R.A., Schenkel, E.P., Barardi, C.R.M., Zanetti, C.R. and Simoes, C.M.O. (2007) Evaluation of Antiviral Activity of South American Plant Extracts against Herpes Simplex Virus Type I and Rabies Virus. Phytotherapy Research, 21, 970-974. https://doi.org/10.1002/ptr.2198
Flores, E.F. (2007) Estrutura e composicao dos vírus. In: Flores, E.F., Eds., Virologia Veterinária, Editora UFSM, Santa Maria/RS, 21-33.
Nolkemper, S., Reichling, J., Stintzing, F.C., Carle, R. and Schnitzler, P. (2006) Antiviral Effect of Aqueous Extracts from Species of the Lamiaceae Family against Herpes Simplex Virus Type 1 and Type 2 in Vitro. Planta Medica, 72, 1378-1382. https://doi.org/10.1055/s-2006-951719
Bakkali, F., Averbeck, S., Averbeck, D. and Idaomar, M. (2008) Biological Effects of Essential Oils: A Review. Food and Chemical Toxicology, 46, 446-475. https://doi.org/10.1016/j.fct.2007.09.106
Koch, C., Reichling, J., Kehm, R., Sharaf, M.M., Zentgraf, H., Schneele, J. and Schnitzler, P. (2008) Efficacy of Anise Oil, Dwarf-Pine Oil and Chamomile Oil against Thymidine-Kinase-Positive and Thymidine-Kinase-Negative Herpesviruses. Journal of Pharmacy and Pharmacology, 60, 1545-1550. https://doi.org/10.1211/jpp.60.11.0017
Saderi, H. and Abbasi, M. (2011) Evaluation of Anti-Adenovirus Activity of Some Plants from Lamiaceae Family Grown in Iran in Cell Culture. African Journal of Biotechnology, 10, 17546-17550.
Neves, J.M., Matos, C., Moutinho, C., Queiroz, G. and Gomes, L.R. (2009) Ethnopharmacological Notes about Ancient Uses of Medicinal Plants in Trás-os-Montes (Northern of Portugal). Journal of Ethnopharmacology, 124, 270-283. https://doi.org/10.1016/j.jep.2009.04.041
Phenolics
Cytotoxicity
Cleff, M.B., Meinerz, A.R., Faria, R.O., Xavier, M.O., Santin, R., Nascente, P.S., Rodrigues, M.R. and Meireles, M.C.A. (2010) Atividade inibitória do óleo essencial de orégano em fungos de importancia médica e veterinária. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62, 1291-1294. https://doi.org/10.1590/S0102-09352010000500040
Sahin, F., Gulluce, M., Daferera, D., Sokmen, A., Sokmen, M., Polissiou, M. and Ozer, H. (2004) Biological Activities of the Essential Oils and Methanol Extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia Region of Turkey. Food Control, 15, 549-557. https://doi.org/10.1016/j.foodcont.2003.08.009
Sarac, N. and Ugur, A. (2008) Antimicrobial Activities of the Essential Oils of Origanum onites L., Origanum vulgare L. Subspecies hirtum (Link) Ietswaart, Satureja thymbra L., and Thymus cilicicus Boiss. & Bal. Growing Wild in Turkey. Journal of Medicinal Food, 11, 568-573. https://doi.org/10.1089/jmf.2007.0520
Kursat, M., Emre, I., Yilmaz, O. and Erecevit, P. (2011) Antioxidant and Antimicrobial Activity in the Seeds of Origanum vulgare L. Subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey. Grasas y Aceites, 62, 410-417. https://doi.org/10.3989/gya.113610
Gulluce, M., Karadayi, M., Guvenalp, Z., Ozbek, H., Arasoglu, T. and Baris, O. (2012) Isolation of Some Active Compounds from Origanum vulgare L. ssp. vulgare and Determination of Their Genotoxic Potentials. Food Chemistry, 130, 248-253. https://doi.org/10.1016/j.foodchem.2011.07.024
Martins, N., Barros, L., Santos-Buelga, C., Henriques, M., Silva, S. and Ferreira, I.C. (2014) Decoction, Infusion and Hydroalcoholic Extract of Origanum vulgare L.: Different Performances Regarding Bioactivity and Phenolic Compounds. Food Chemistry, 158, 73-80. https://doi.org/10.1016/j.foodchem.2014.02.099
Bendifallah, L., Tchoulak, Y., Djouabi, M., Oukili, M. and Ghezraoui, R. (2015) Phytochemical Study and Antimicrobial Activity of Origanum vulgare L. (Lamiaceae) in Boumerdes Mountainous Region (Algeria). Journal of Medical and Bioengineering, 4, 471-474. https://doi.org/10.12720/jomb.4.6.471-474
Singletary, K. (2010) Oregano: Overview of the Literature on Health Benefits. Nutrition Today, 45, 129-138. https://doi.org/10.1097/NT.0b013e3181dec789
Zhang, X.L., Guo, Y.S., Wang, C.H., Li, G.Q., Xu, J.J., Chung, H.Y., Ye, W.-C., Li, Y.-L. and Wang, G.C. (2014) Phenolic Compounds from Origanum vulgare and Their Antioxidant and Antiviral Activities. Food Chemistry, 152, 300-306. https://doi.org/10.1016/j.foodchem.2013.11.153
Yan, F., Azizi, A., Janke, S., Schwarz, M., Zeller, S. and Honermeier, B. (2016) Antioxidant Capacity Variation in the Oregano (Origanum vulgare L.) Collection of the German National Genebank. Industrial Crops and Products, 92, 19-25. https://doi.org/10.1016/j.indcrop.2016.07.038
Morshedloo, M.R., Craker, L.E., Salami, A., Nazeri, V., Sang, H. and Maggi, F. (2017) Effect of Prolonged Water Stress on Essential Oil Content, Compositions and Gene Expression Patterns of Mono- and Sesquiterpene Synthesis in Two Oregano (Origanum vulgare L.) Subspecies. Plant Physiology and Biochemistry, 111, 119-128. https://doi.org/10.1016/j.plaphy.2016.11.023
Skerget, M., Kotnik, P., Hadolin, M., Hras, A.R., Simonic, M. and Knez, Z. (2005) Phenols, Proanthocyanidins, Flavones and Flavonols in Some Plant Materials and Their Antioxidant Activities. Food Chemistry, 89, 191-198. https://doi.org/10.1016/j.foodchem.2004.02.025
Mueller, M., Lukas, B., Novak, J., Simoncini, T., Genazzani, A.R. and Jungbauer, A. (2008) Oregano: A Source for Peroxisome Proliferator-Activated Receptor Antagonists. Journal of Agricultural and Food Chemistry, 56, 11621-11630. https://doi.org/10.1021/jf802298w
Snijder, E.J. and Meulenberg, J.J. (1998) The Molecular Biology of Arteriviruses. Journal of General Virology, 79, 961-979. https://doi.org/10.1099/0022-1317-79-5-961
Mosmann, T. (1983) Rapid Colorimetric Assay for Cellular Growth and Survival. Journal of Immunological Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
Mayr, A., Bachmann, P.A., Bibrack, B.M. and Wittmann, G. (1982) Virologische Arbeitsmethoden-Band IV-Sicherheit bei virologischen arbeiten-Biometrische Methoden. Gustav Fischer Verlag, Stutgart.
Danila, O.A., Gatea, F. and Radu, G.L. (2011) Polyphenol Composition and Antioxidant Activity of Selected Medicinal Herbs. Chemistry of Natural Compounds, 47, 22-26. https://doi.org/10.1007/s10600-011-9822-7
Tafuri, N.F. (2011) Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1). Dissertacao (Mestrado em Bioquímica agrícola) Universidade de Vicosa, Minas Gerais.
Schnitzler, P., Nolkemper, S., Stintzing, F.C. and Reichling, J. (2008) Comparative in Vitro Study on the Anti-Herpetic Effect of Phytochemically Characterized Aqueous and Ethanolic Extracts of Salvia officinalis Grown at Two Different Locations. Phytomedicine, 15, 62-70. https://doi.org/10.1016/j.phymed.2007.11.013
Khan, M.T.H., Ather, A., Thompson, K.D. and Gambari, R. (2005) Extracts and Molecules from Medicinal Plants against Herpes Simplex Viruses. Antiviral Research, 67, 107-119. https://doi.org/10.1016/j.antiviral.2005.05.002
Cotin, S., Calliste, C., Mazeron, M., Hantz, S., Duroux, J., Rawlinson, W., Ploy, M. and Alain, S. (2012) Eight Flavonoids and Their Potential as Inhibitors of Human Cytomegalovirus Replication. Antiviral Research, 96, 181-1866. https://doi.org/10.1016/j.antiviral.2012.09.010
Wang, J.N., Hou, C.Y., Liu, Y.L., Lin, L.Z., Gil, R.R. and Cordell, G.A. (1994) Swertifrancheside, an HIV-Reverse Transcriptase Inhibitor and the First Flavone-Xanthone Dimmer, from Swertia Franchetiana. Journal of Natural products, 57, 211-217. https://doi.org/10.1021/np50104a003
Sy, L., Rhim, J.Y. and Park, W.B. (2005) Antiherpetic Activities of Flavonoids against Herpes Simplex Virus Type 1(HSV-1) and Type 2 (HSV-2) in Vitro. Archives of Pharmacology Research, 28, 1293-1301. https://doi.org/10.1007/BF02978215
Formica, J.V. and Regelson, W. (1995) Review of Biology of Quercetin and Related Bioflavanoids. Food Chemical Toxicology, 33, 1061-1080. https://doi.org/10.1016/0278-6915(95)00077-1