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Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i> (L.) Verdc.) in Burkina Faso

¹ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
² Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
³ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁴ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁵ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁶ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁷ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁸ Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA, Ouagadougou, Burkina Faso
⁹ Université Ouaga I Professeur Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
¹⁰ Laboratoire National de Biosécurité, Ouagadougou, Burkina Faso

Advances in Bioscience and Biotechnology·Volume 10 (2019)·Pages 270–281·Published 17 September 2019·DOI10.4236/abb.2019.109021

Abstract

Potyviruses are major constraints to grain legume production by causing significant yield losses. Potyviruses infecting Bambara groundnut ( Vigna subterranea ) were investigated in Burkina Faso. Leaf samples collected from three agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using potyvirus universal primers. Analysis of full coat protein (cp) sequences from 24 isolates revealed the occurrence of three groups of Bambara groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5 % - 100% nucleotide ( nt ) identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV) and Passiflora virus Ugandan which were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8 % - 79.9% aa identity with BCMNV and group 3 shared 77.3 % - 78.3% nt and 80.7 % - 81.5% aa identity with Passiflora virus Ugandan . All three groups were confirmed by phylogenetic analyses. Taking into account potyvirus demarcation criteria, group 1 isolates belonged to CABMV species. Group 2 and group 3 were assigned to a potentially new potyviruses species and designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).

KeywordsBambara GroundnutPotyvirusCharacterizationCoat Protein SequenceDiversity

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Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i> (L.) Verdc.) in Burkina Faso

Abstract

References (20)