Intrinsic Bio-Enhancer Entities of <i>Fagonia cretica</i> for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity — Oak Academic Publishing
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Intrinsic Bio-Enhancer Entities of <i>Fagonia cretica</i> for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
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Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
,
Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
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Department of Chemistry, The Islamia University, Bahawalpur, Pakistan
,
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
,
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
,
Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
,
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
,
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
,
Cholistan Institute of Desert Studies (CIDS), The Islamia University, Bahawalpur, Pakistan
,
Department of Botany, The Islamia University, Bahawalpur, Pakistan
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College of Information Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, China
,
Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
1 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
2 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
3 Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
4 Department of Chemistry, The Islamia University, Bahawalpur, Pakistan
5 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
6 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
7 Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
8 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
9 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
10 Cholistan Institute of Desert Studies (CIDS), The Islamia University, Bahawalpur, Pakistan
11 Department of Botany, The Islamia University, Bahawalpur, Pakistan
12 College of Information Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, China
13 Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan
Objective: Evaluating the bio-reducing activity and various potential, annotates the biological entitties of Fagonia cretica. Method: By fractionating the crude extract with different ethanol-water fraction mixture. This substantiates the reduction potential for the biological synthesis of AgNPs, its antioxidant, anti-urease and anti-Tyrosinase activity. Results: UV-visible spectra confirm the bio-reducing potential for Ag NPs with the optimized 50% ethanol-water fraction mixture. Transmission electron microscopy (TEM) confirmed the round shaped AgNPs with an average size of 16 nm in optimized 50% fraction mixture. Further evaluating Ethanol-water fraction with 90% and 70% of plant extract showed more potentials for Anti-Tyrosinase and Anti-urease activity. Moreover, diluted 30% fraction exhibits two folds higher Anti-oxidant and Anti urease activity. Conclusion: Overall, this work showed that 50% Ethanol-water fraction has potentially active molecules (among all fractions) primarily involved in bio-reducing potential of Ag NPs and enzymatic assay while 90% mixture proved to be least active. This study suggested electing the optimum mixture fraction of 50% ethanol-water in order to screen the bioactive compounds and to inculcate their activity status for clinical trials.
Hasan, M., Ullah, I., et al. (2018) Biological Entities as Chemical Reactors for Synthesis of Nanomaterials: Progress, Challenges and Future Perspective. Materials Today Chemistry, 8, 13-28. https://doi.org/10.1016/j.mtchem.2018.02.003
Hasan, M., Teng, Z.Q., Iqbal, J., Awan, U., et al. (2013) Assessment of Bioreducing and Stabilizing Potential of Dragon’s Blood (Dracaena Cochinchinensis, Lour. S. C. Chen) Resin Extract in Synthesis of Silver Nanoparticles. Nanoscience and Nanotechnology Letters, 5, 780-784. https://doi.org/10.1166/nnl.2013.1600
Dang, H., Hasan, M., et al. (2014) Luteolin-Loaded Solid Lipid Nanoparticles Synthesis, Characterization, & Improvement of Bioavailability, Pharmacokinetics in Vitro and Vivo Studies. Journal of Nanoparticle Research, 16, Article ID: 2347.
Hasan, M., Iqbal, J., Awan, U., Xin, N., Dang, H., et al. (2015) LX Loaded Nanoliposomes Synthesis, Characterization and Cellular Uptake Studies in H2O2 Stressed SH-SY5Y Cells. Journal of Nanoscience and Nanotechnology, 15, 1320-1326.
Hasan, M., Mustafa, G., Iqbal, J., Ashfaq, M. and Mahmood, N. (2018) Quantitative Proteomic Analysis of HeLa Cells in Response to Biocompatible Fe2C@C Nanoparticles: 16O/18O-Labelling & HPLC-ESI-Orbit-Trap Profiling Approach. Toxicological Research, 7, 84-92. https://doi.org/10.1039/C7TX00248C
Hasan, M., et al. (2017) Biocompatibility of Iron Carbide and Detection of Metals Ions Signaling Proteomic Analysis via HPLC/ESI-Orbitrap. Nano Research, 10, 1912-1923. https://doi.org/10.1007/s12274-016-1375-4
Akbar, S., et al. (2018) Raphanus Sativus Mediated Synthesis, Characterization and Biological Evaluation of Zinc Oxide Nanoparticles. Nanoscience and Nanotechnology Letters, 9, 2005-2012. https://doi.org/10.1166/nnl.2017.2550
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G. and Lightfoot, D.A. (2017) Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants, 6, 23-42. https://doi.org/10.3390/plants6040042
Qureshi, H., Asif, S., Ahmed, H., Al-Kahtani, H.A. and Hayat, K. (2016) Chemical Composition and Medicinal Significance of Fagonia cretica: A Review. Natural Product Research, 30, 625-639. https://doi.org/10.1080/14786419.2015.1036268
Farheen, R., Mahmood, I. and Parveen, R. (2017) Review on Medicinal and Bioactive Role of Genus Fagonia. FUUAST Journal of Biology, 7, 33-36.
Naeem, K., Yawar, W., Muhammad, B. and Rehana, I. (2014) Assessment of Macronutrients and Heavy Metals in Fgoniacretica Linn of Pakistan by Atomic Spectroscopy. Bulletin of the Chemical Society of Ethiopia, 28, 177-185. https://doi.org/10.4314/bcse.v28i2.2
Nazir, I., Ur Rahman, N., Alvi, Z., Hafizur Rahman, M., et al. (2017) Antidiabetic Activities of an LC/MS Fingerprinted Aqueous Extract of Fagonia cretica L. in Preclinical Models. Planta Medica, 83, 1141-1148. https://doi.org/10.1055/s-0043-107616
Hussain, A., Zia, M. and Mirza, B. (2007) Cytotoxic and Antitumor Potential of Fagonia cretica L. Turkish Journal of Biology, 31, 19-24.
Chopra, R., Chopra, I., et al. (1982) Indigenous Drugs of India. 2nd Edition, Academic Publishers, Calcutta.
Marwat, S.K., Fazal-Ur-Rehman and Khan, I.U. (2012) Tracing the Useful Ethnophytomedicinal Recipes of Angiosperms Used against Jaundice and Hepatitis in Indo-Pak Subcontinent. World Applied Sciences Journal, 18, 1243-1252.
Iqbal, P., Ahmed, D. and Asghar, M.N. (2014) A Comparative in Vitro Antioxidant Potential Profile of Extracts from Different Parts of Fagonia cretica. Asian Pacific Journal of Tropical Medicine, 7, S473-S480. https://doi.org/10.1016/S1995-7645(14)60277-7
Iqbal, H., Sher, Z. and Khan, Z.U. (2011) Medicinal Plants from Salt Range Pind Dadan Khan, District Jhelum, Punjab, Pakistan. Journal of Medicinal Plants Research, 5, 2157-2168.
Badshah, L. and Hussain, F. (2011) People Preferences and Use of Local Medicinal Flora in District Tank, Pakistan. Journal of Medicinal Plants Research, 5, 22-29.
Satpute, R., Bhattacharya, R., Kashyap, R.S., et al. (2012) Antioxidant Potential of Fagonia arabica against the Chemical Ischemia-Unduced in PC12 Cells. Iranian Journal of Pharmaceutical Research, 11, 303-313.
Iqbal, P., Ahmed, D. and Asghar, M.N. (2014) A Comparative in Vitro Antioxidant Potential Profile of Extracts from Different Parts of Fagonia cretica. Asian Pacific Journal of Tropical Medicine, 7, S473-S480. https://doi.org/10.1016/S1995-7645(14)60277-7
Rawal, A.K., Nath, D.K., Yadav, N., Pande, S., Meshram, S.U. and Biswas, S.K. (2009) Rubiacordifolia, Fagonia cretica Linn and Tinospora cordifolia Exert Anti-Inflammatory Properties by Modulating Platelet Aggregation and VEGF, COX-2 and VCAM Gene Expressions in Rat Hippocampal Slices Subjected to Ischemic Reperfusion Injury. International Journal of Applied Research in Natural Products, 2, 19-26.
Ran, Y., Wang, R., Lin, F., Hasan, M., Jia, Q., Tang, B., et al. (2014) Radio Protective Effects of Dragon’s Blood and Its Extract against Gamma Irradiation in Mouse Bone Marrow Cells. Physics in Medicine, 30, 427-431. https://doi.org/10.1016/j.ejmp.2013.12.001
Deghbar, N., Mezioug, D., Kahina, T., Medjdoub, Y.M., et al. (2019) Antihydatic and Immunomodulatory Effects of Algerian Propolisethanolic Extract: In Vitro and in Vivo Study. Asian Pacific Journal of Tropical Medicine, 12, 106-116.
Ran, Y., Wang, R., Gao, Q., Jia, Q., Hasan, M., Awan, M.U., et al. (2014) Dragon’s Blood and Its Extracts Attenuate Radiation-Induced Oxidative Stress in Mice. Journal of Radiation Research, 55, 699-706. https://doi.org/10.1093/jrr/rru013
Ran, Y., Xu, B., Wang, R., Gao, Q., Jia, Q., Hasan, M., et al. (2016) Dragon’s Blood Extracts Reduce Radiation-Induced Peripheral Blood Injury and Protects Human Megakaryocyte Cells from GM-CSF Withdraw-Induced Apoptosis. Physica Medica, 32, 84-93.
Mahmood, S., Fatima, T., Zulfaqar, H., Saher, R., et al. (2017) Meta-Analysis of Dragon’s Blood Resin Extract as Radio-Protective Agent. Journal of Coastal Life Medicine, 5, 409-416.
Touzani, S., Al-Waili, N., El Menyiy, N., Filipic, B., et al. (2018) Chemical Analysis and Antioxidant Content of Various Propolis Samples Collected from Different Regions and Their Impact on Antimicrobial Activities. Asian Pacific Journal of Tropical Medicine, 11, 436-442.
Uddin, Md.S., Hossain, Md.S., Al Mamun, A., et al. (2018) Phytochemical Analysis and Antioxidant Profile of Methanolic Extract of Seed, Pulp and Peel of Baccaurea ramiflora Lour. Asian Pacific Journal of Tropical Medicine, 11, 443-450.
Xin, N., Hasan, M., Li, W. and Li, Y. (2014) Juglans mandshurica Maxim Extracts Exhibit Antitumor Activity on HeLa Cells in Vitro. Molecular Medicine Reports, 9, 1313-1318. https://doi.org/10.3892/mmr.2014.1979
Ozer, O., Berkay, M. and Bijan, K. (2007) Antityrosinase Activity of Some Plant Extracts and Formulations Containing Ellagic Acid. Pharmaceutical Biology, 45, 519-524. https://doi.org/10.1080/13880200701446746
Mobley, H. and Hausinger, R. (1989) Microbial Ureases: Significance, Regulation, and Molecular Characterization. Microbiological Reviews, 53, 85-108.
Iqbal, H. and Sher, Z. (2011) Medicinal Plants from Salt Range Pind Dadan Khan, District Jhelum, Punjab, Pakistan. Journal of Medicinal Plant Research, 5, 2157-2168.
Cáceres, A., Almeda, F., et al. (2018) Anti-Urease Activity of Native Species of Genus Piper from Guatemala with Potential Application in Infection Control. International Journal of Phytocosmetics and Natural Ingredients, 5, 2. https://doi.org/10.15171/ijpni.2018.02
Azmi, N., Hashim, P., Hashim, D.M., Halimoon, N. and Majid, N. (2014) Anti-Elastase, Anti-Tyrosinase and Matrix Metalloproteinase-1 Inhibitory Activity of Earthworm Extracts as Potential New Anti-Aging Agent. Asian Pacific Journal of Tropical Medicine, 4, 348-352. https://doi.org/10.12980/APJTB.4.2014C1166
Baurin, N., Arnoult, E., Scior, T., Do, Q. and Bernard, P. (2002) Preliminary Screening of Some Tropical Plants for Antityrosinase Activity. Journal of Ethnopharmacology, 82, 155-158. https://doi.org/10.1016/S0378-8741(02)00174-5
Lateef, M., Iqbal, L., Fatima, N., Siddiqui, K., Afza, N., et al. (2012) Evaluation of Antioxidant and Urease Imbibition Activities of Roots of Glycyrrihza glabra. Pakistan Journal of Pharmaceutical Sciences, 25, 99-102.
Saadullah, M., Chaudary, B.A. and Uzair, M. (2016) Antioxidant Phytotoxic and Antiurease Activities and Total Phenolic and Flavonoid Contents of Conocorpus lancifolius. Tropical Journal of Pharmaceutical Research, 15, 555-561. https://doi.org/10.4314/tjpr.v15i3.17
Nile, S.H., Keum, Y.S., Nile, A.S., Jalde, S.S. and Patel, R.V. (2017) Antioxidant Anti-Inflammatory and Enzyme Inhibitory Activity of Natural Plant Flavonoid and Their Synthesised Derivatives. Journal of Biochemical and Molecular Toxicology, 32, e22002. https://doi.org/10.1002/jbt.22002
Zengin, G., Uysal, S., Ceylan, R. and Aktumsek, A. (2015) Phenolic Constituents Antioxidative and Tyrosinase Inhibitory Activity of Ornithogalum narbonense L. from Turkey: A Phytochemical Study. Industrial Crops and Products, 70, 1-6. https://doi.org/10.1016/j.indcrop.2015.03.012
Athipornchai, A. and Jullapo, A. (2018) Tyrosinase Inhibitory and Antioxidant Activities of Orchid (Dendrobium spp.). South African Journal of Botany, 119, 188-192. https://doi.org/10.1016/j.sajb.2018.09.003