Mild hydrochloric acid hydrolysis of i -carrageenan from Eucheuma spinosum yielded two oligosaccharides of sulfated tetrasaccharide structure. These were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance (NMR) and Electrospray Ionization Mass Spectrometry (ESIMS). Both oligosaccharides have structure of b -D-galactopyranose(Gal p )4S-(1 → 4)- α -D-AnGal p 2S-(1 → 3)- b -D-galactopyranose Gal p )4S-(1 → 4)- α -D-AnGal p 2S -(1 → 3). Application of the resulting oligosaccharides on protein delivery system in terms of encapsulation efficiency was performed.
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