Portable Diagnostic Platform for Detection of Microorganisms Coliforms and <i>E. coli</i> — Oak Academic Publishing
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Portable Diagnostic Platform for Detection of Microorganisms Coliforms and <i>E. coli</i>
Rede de Biodiversidade e Biotecnologia da Amazônia Legal (BIONORTE), Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
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Department of Chemistry, Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
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Department of Chemistry, Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
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Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Belém, Brazil
1 Rede de Biodiversidade e Biotecnologia da Amazônia Legal (BIONORTE), Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
2 Department of Chemistry, Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
3 Department of Chemistry, Fundação Universidade Federal de Rondônia (UNIR), Porto Velho, Brazil
4 Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Belém, Brazil
Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.
Berthier, J., Brakke, K.A. and Berthier, E. (2016) Paper-Based Microfluidics. In: Open Microfluidics, John Wiley and & Sons, Hoboken, 229-256. https://doi.org/10.1002/9781118720936.ch7
Burgess, D.C.H., Wasserman, J. and Dahl, C.A. (2006) Global Health Diagnostics. Nature, SI, 1-2. https://doi.org/10.1038/nature05440
Peeling, R.W. and Mabey, D. (2010) Point-of-Care Tests for Diagnosing Infections in the Developing World. Clinical Microbiology and Infection, 16, 1062-1069. https://doi.org/10.1111/j.1469-0691.2010.03279.x
Urdea, M., Penny, L.A., Olmsted, S.S., Giovanni, M.Y., Kaspar, P., Shepherd, A., Wilson, P., Dahl, C.A., Buchsbaum, S., Moeller, G. and Hay Burgess, D.C. (2006) Requirements for High Impact Diagnostics in the Developing World. Nature, 444, 73-79. https://doi.org/10.1038/nature05448
Sharma, S., Zapa-tero-Rodríguez, J., Estrela, P. and O’Kennedy, R. (2015) Point-of-Care Diagnostics in Low Resource Settings: Present Status and Future Role of Microfluidics. Biosensors, 5, 577-601. https://doi.org/10.3390/bios5030577
Martinez, A.W., Phillips, S.T., Carrilho, E., Thomas, S.W., Sindi, H. and Whitesides, G.M. (2008) Simple Telemedicine for Developing Regions: Camera Phones and Paper-Based Microfluidic Devices for Real-Time, Off-Site Diagnosis. Analytical Chemistry, 80, 3699-3707. https://doi.org/10.1021/ac800112r
Carrilho, E., Martinez, A.W. and Whitesides, G.M. (2009) Understanding Wax Printing: A Simple Micropatterning Process for Paper-Based Microfluidics. Analytical Chemistry, 81, 7091-7095. https://doi.org/10.1021/ac901071p
Zhao, W. and Van Den Berg, A. (2008) Lab on Paper. Lab on a Chip, 8, 1988-1991. https://doi.org/10.1039/b814043j
Martinez, A.W., Phillips, S.T., Whitesides, G.M. and Carrilho, E. (2010) Diagnostics for the Developing World: Microfluidic Paper-Based Analytical Devices. Analytical Chemistry, 82, 3-10. https://doi.org/10.1021/ac9013989
Credou, J. and Berthelot, T. (2014) Cellulose: From Biocompatible to Bioactive Material. Journal of Materials Chemistry B, 2, 4767-4788. https://doi.org/10.1039/C4TB00431K
Pruss-Ustun, A., Bonjour, S. and Corvalán, C. (2008) The Impact of the Environment on Health by Country: A Meta-Synthesis. Environmental Health, 7, 7. https://doi.org/10.1186/1476-069X-7-7
Vasavada, P.C. (1993) Rapid Methods and Automation in Dairy Microbiology. Journal of Dairy Science, 76, 3101-3113. https://doi.org/10.3168/jds.S0022-0302(93)77649-3
Tavolaro, P., Ferrati, A.R., Destro, M.T., Landgraf, M. and De Melo Franco, B.D.G. (2005) Performance of Two Ready-to-Use Systems for Enumeration of Aerobic Mesophilic Microorganisms in Frozen Goat Milk. Brazilian Journal Microbiology, 36, 295-300. https://doi.org/10.1590/S1517-83822005000300017
Hossain, S.M.Z., Ozimok, C., Sicard, C., Aguirre, S.D., Ali, M.M., Li, Y. and Brennan, J.D. (2012) Multiplexed Paper Test Strip for Quantitative Bacterial Detection. Analytical and Bioanalytical Chemistry, 403, 1567-1576. https://doi.org/10.1007/s00216-012-5975-x
Ma, S., Tang, Y., Liu, J. and Wu, J. (2014) Visible Paper Chip Immunoassay for Rapid Determination of Bacteria in Water Distribution System. Talanta, 120, 135-140. https://doi.org/10.1016/j.talanta.2013.12.007
Gunda, N.S.K., Dasgupta, S. and Mitra, S.K. (2017) DipTest: A Litmus Test for E. coli Detection in Water. PLoS ONE, 12, e0183234. https://doi.org/10.1371/journal.pone.0183234
Pessoa, G.V.A. and Silva, E.A. (1972) Meios de Rugai e Lisinamotilidade combinados em um só tubo para a identificação presuntiva de enterobactérias. Instituto Adolpho Lutz, 32, 97-100.
NCCLS (2005) Metodologia dos Testes de Sensibilidade a Agentes Antimicrobianos por Diluição para Bactéria de Crescimento Aeróbico. Norma Aprovada, 23, 1-49. http://bvsms.saude.gov.br/bvs/publicacoes/metodo_ref_testes_diluicao_modulo3.pdf
BRASIL (2017) Resolução RDC no 166 de 24 de junho de 2017. Dispõe sobre a Validação de métodos analíticos e dá outras providências. Agência Nacional Vigilancia sanitária. http://www.in.gov.br/materia/-/asset_publisher/Kujrw0TZC2Mb/content/id/19194581/do1-2017-07-25-resolucao-rdc-n-166-de-24-de-julho-de-2017-19194412
Funes-Huacca, M., Wu, A., Szepesvari, E., Rajendran, P., Kwan-Wong, N., Razgulin, A., Shen, Y., Kagira, J., Campbell, R. and Derda, R. (2012) Portable Self-Contained Cultures for Phage and Bacteria Made of Paper and Tape. Lab on a Chip, 12, 4269-4278. https://doi.org/10.1039/c2lc40391a
Drain, P.K., Hyle, E.P., Noubary, F., Freedberg, K.A., Wilson, D., Rodriguez, W. and Bassett, I.V. (2014) Evaluating Diagnostic Point-of-Care Tests in Resource-Limited Settings. The Lancet Infectious Diseases, 14, 239-249. https://doi.org/10.1016/S1473-3099(13)70250-0
Su, W., Gao, X., Jiang, L. and Qin, J. (2015) Microfluidic Platform towards Point-of-Care Diagnostics in Infectious Diseases. Journal of Chromatography A, 1377, 13-26. https://doi.org/10.1016/j.chroma.2014.12.041
Mabey, D., Peeling, R.W., Ustianowski, A. and Perkins, M.D. (2004) Diagnostics for the Developing World. Nature Reviews Microbiology, 2, 231-240. https://doi.org/10.1038/nrmicro841
Yager, P., Domingo, G.J. and Gerdes, J. (2008) Point-of-Care Diagnostics for Global Health. Annual Review of Biomedical Engineering, 10, 107-144. https://doi.org/10.1146/annurev.bioeng.10.061807.160524
Martinez, A.W., Phillips, S.T., Butte, M.J. and Whitesides, G.M. (2007) Patterned Paper as a Platform for Inexpensive, Low-Volume, Portable Bioassays. Angewandte Chemie International Edition, 46, 1318-1320. https://doi.org/10.1002/anie.200603817
Derda, R., Laromaine, A., Mammoto, A., Tang, S.K.Y., Mammoto, T., Ingber, D.E. and Whitesides, G.M. (2009) Paper-Supported 3D Cell Culture for Tissue-Based Bioassays. Proceedings of the National Academy of Sciences of the United States of America, 106, 18457-18462. https://doi.org/10.1073/pnas.0910666106
Ellerbee, A.K., Phillips, S.T., Siegel, A.C., Mirica, K.A., Martinez, A.W., Striehl, P., Jain, N., Prentiss, M. and Whitesides, G. (2009) Quantifying Colorimetric Assays in Paper-Based Microfluidic Devices by Measuring the Transmission of Light through Paper. Analytical Chemistry, 81, 8447-8452. https://doi.org/10.1021/ac901307q
Dungchai, W., Chailapakul, O. and Henry, C.S. (2009) Electrochemical Detection for Paper-Based Microfluidics. Analytical Chemistry, 81, 5821-5826. https://doi.org/10.1021/ac9007573
Yu, J., Ge, L., Huang, J., Wang, S. and Ge, S. (2011) Microfluidic Paper-Based Chemiluminescence Biosensor for Simultaneous Determination of Glucose and Uric Acid. Lab on a Chip, 11, 1286-1291. https://doi.org/10.1039/c0lc00524j
Fang, X., Chen, H., Jiang, X. and Kong, J. (2011) Microfluidic Devices Constructed by a Marker Pen on a Silica Gel Plate for Multiplex Assays. Analytical Chemistry, 83, 3596-3599. https://doi.org/10.1021/ac200024a
Nie, Z., Nijhuis, C.A., Gong, J., Chen, X., Kumachev, A., Martinez, A.W., Narovlyansky, M. and Whitesides, G.M. (2010) Electrochemical Sensing in Paper-Based Microfluidic Devices. Lab on a Chip, 10, 477-483. https://doi.org/10.1039/B917150A
Lu, J., Ge, S., Ge, L., Yan, M. and Yu, J. (2012) Electrochemical DNA Sensor Based on Three-Dimensional Folding Paper Device for Specific and Sensitive Point-of-Care Testing. Electrochimica Acta, 80, 334-341. https://doi.org/10.1016/j.electacta.2012.07.024
Haga, S.B. (2016) Challenges of Development and Implementation of Point of Care Pharmacogenetic Testing. Expert Review of Molecular Diagnostics, 16, 949-960. https://doi.org/10.1080/14737159.2016.1211934
Derda, R., Tang, S.K.Y., Laromaine, A., Mosadegh, B., Hong, E., Mwangi, M., Mammoto, A., Ingber, D.E. and Whitesides, G.M. (2011) Multizone Paper Platform for 3D Cell Cultures. PLoS ONE, 6, e18940. https://doi.org/10.1371/journal.pone.0018940
Dungchai, W., Chailapakul, O. and Henry, C.S. (2011) A Low-Cost, Simple, and Rapid Fabrication Method for Paper-Based Microfluidics Using Wax Screen-Printing. Analyst, 136, 77-82. https://doi.org/10.1039/C0AN00406E
Zamora, V., Marx, S., Arndt-Staufenbiel, N., Janeczka, C., Havlik, G., Queisser, M. and Schröder, H. (2017) Laser-Microstructured Double-Sided Adhesive Tapes for Integration of a Disposable Biochip. Proceedings, 1, 606. https://doi.org/10.3390/proceedings1040606
Kudo, H., Sawada, T., Kazawa, E., Yoshida, H., Iwasaki, Y. and Mitsubayashi, K. (2006) A Flexible and Wearable Glucose Sensor Based on Functional Polymers with Soft-MEMS Techniques. Biosensors and Bioelectronics, 22, 558-562. https://doi.org/10.1016/j.bios.2006.05.006
Manafi, M. (2000) New Developments in Chromogenic and Fluorogenic Culture Media. International Journal of Food Microbiology, 60, 205-218. https://doi.org/10.1016/S0168-1605(00)00312-3
Manafi, M. (2003) Chapter 12 Media for Detection and Enumeration of “Total” Enterobacteriaceae, Coliforms and Escherichia coli from Water and Foods. Progress in Industrial Microbiology, 37, 167-193. https://doi.org/10.1016/S0079-6352(03)80015-2
Madmanang, R., He, Z. and Sriwiriyarat, T. (2018) Respirometric Activities of Unacclimatized Enterobacter aerogenes and Mixed Culture Bacteria in Sequencing Batch Reactor Systems in Response to Acrylamide and Its Biodegradation Products. Royal Society Chemistry, 8, 34911-34920. https://doi.org/10.1039/C8RA06668J