This study aimed to evaluate the antimicrobial activity of the extract of Libidibia ferrea L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of Libidibia ferrea L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL ( Streptococcus mutans ), 3.750 μg/mL ( Streptococcus oralis ), 4.375 μg/mL ( Lactobacillus casei ). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against Streptococcus mutans and bactericidal against Streptococcus oralis ). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for Streptococcus mutans and Lactobacillus casei , respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for Streptococcus mutans , and 9.60% for Lactobacillus casei were observed. It can be concluded that the extract and its mouthwash containig Libidibia ferrea L. showed antibacterial activity against microorganisms from the dental biofilm.
KeywordsMouthwashJucaNatural Products
Marsh, P.D. and Zaura, E. (2017) Dental Biofilm: Ecological Interactions in Health and Disease. Journal of Clinical Periodontology, 44, 12-22. https://doi.org/10.1111/jcpe.12679
Moro, I.S., Gondo, G.D.G.A., Pierri, E.G., Pietro, R.C.L.R., Soares, C.P., Sousa, D.P., et al. (2017) Evaluation of Antimicrobial, Cytotoxic and Chemopreventive Activities of Carvone and Its Derivatives. Brazilian Journal of Pharmaceutical Sciences, 53, 1-8. https://doi.org/10.1590/s2175-97902017000400076
Barreto, B.B. and Vieira, R.C.P.A. (2015) Perception of Health Professionals about the Inclusion of Phytotherapy in Primary Health Care. Revista de Atenção Primária à Saúde, 18, 191-198.
Santos, R.L., Guimaraes, G.P., Nobre, M.S.C. and Portela, A.S. (2011) Analysis of Phytotherapy as an Integrative Practice in the Unified Health System. Revista Brasileira de Plantas Medicinais, 13, 486-491. https://doi.org/10.1590/S1516-05722011000400014
Oliveira, G.P., Souza, T.P., Caetano, S.K., Farias, K.S., Venancio, G.N., Bandeira, M.F.C.L., et al. (2013) Antimicrobial Activity in Vitro of Extracts of the Stem Bark and Fruit of Libidibia ferrea L. against Microorganisms of the Oral Cavity. Revista Fitos, 8, 73-160. https://doi.org/10.5935/1808-9569.20130004
Simões, C.A.C.G., Conde, N.C.O., Venancio, G.N., Milério, P.S.L.L., Bandeira, M.F.C.L. and Veiga Júnior, V.F. (2016) Antibacterial Activity of Copaiba Oil Gel on Dental Biofilm. Open Dentistry Journal, 10, 188-195. https://doi.org/10.2174/1874210601610010188
Vasconcelos, K.R.F., Veiga Junior, V.F., Rocha, W.C. and Bandeira, M.F.C.L. (2008) In Vitro Assessment of the Antibacterial Activity of a Dental Cement Constituted of a Copaifera multijuga Hayne Oil-Resin. Revista Brasileira de Farmacognosia, 18, 733-738. https://doi.org/10.1590/S0102-695X2008000500017
Sampaio, F.C., Pereira, M.S., Dias, C.S., Costa, V.C., Conde, N.C. and Buzalaf, M.A. (2009) In Vitro Antimicrobial Activity of Caesalpinia ferrea Martius Fruits against Oral Pathogens. Journal of Ethnopharmacology, 124, 289-294. https://doi.org/10.1016/j.jep.2009.04.034
Freitas, A.C.C., Ximenes, N.C.A., Aguiar, J.S., Nascimento, S.C., Lins, T.U.L., Magalhães, L.R. et al. (2012) Biological Activies of Libidibia (Caesalpinia) ferrea var. parvifolia (Mart. ex Tul.) L.P. Queiroz Pod Preparations. Evidence-Based Complementary and Alternative Medicine, 2012, Article ID: 514134. https://doi.org/10.1155/2012/514134
Pedrosa, T.N., Barros, A.O., Nogueira, J.R., Fruet, A.C., Rodrigues, I.C., Calcagno, D.Q., et al. (2016) Anti-Wrinkle and Anti-Whitening Effects of jucá (Libidibia ferrea Mart.) Extracts. Archives of Dermatological Research, 308, 643-654. https://doi.org/10.1007/s00403-016-1685-0
Ferreira, M.R.A., Fernandes, M.T.M., Silva, W.A.V., Bezerra, I.C.F., de Souza, T.P., Pimentel, M.F., et al. (2016) Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius. Pharmacognosy Magazine, 12, 285-291. https://doi.org/10.4103/0973-1296.182165
Zanin, S.M.W., Miguel, M.D., Barreira, S.M.W., Nakashima, T., Cury, C.D. and Costa, C.C. (2007) Mouthwasher: Active Principles and Development of a Formula with Hydroalcoholic Extract of Salvia officinalis L. Visão Acadêmica, 8, 19-24. https://doi.org/10.5380/acd.v8i1.11661
NCCLS (2003) Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eighth Edition. NCCLS Document M2-A8, NCCLS, Wayne, Pennsylvania.
Andrews, J.M. (2007) Determination of Minimum Inhibitory Concentrations. Journal of Antimicrobial Chemotherapy, 41, 5-16. https://doi.org/10.1093/jac/48.suppl_1.5
Filoche, S.K., Coleman, M.J. and Sissons, C.H. (2007) A Fluorescence Assay to Determine the Viable Biomass of Microcosm Dental Plaque Biofilms. Journal of Microbiological Methods, 69, 489-496. https://doi.org/10.1016/j.mimet.2007.02.015
Vieira, S. (2004) Biostatistics, Advanced Topics. 2nd Edition, Elsevier, Rio de Janeiro.
Marreiro, R.O., Bandeira, M.F.C.L., Souza, T.P., Almeida, M.C., Bendaham, K., Venancio, G.N., et al. (2014) Evaluation of the Stability and Antimicrobial Activity of an Ethanolic Extract of Libidibia ferrea. Clinical, Cosmetic and Investigational Dentistry, 6, 9-13. https://doi.org/10.2147/CCIDE.S54319
Robertson, J., McGoverin, C., Vanholsbeeck, F., Swift, S. (2019) Optimization of the Protocol for the LIVE/DEAD R BacLightTM Bacterial Viability Kit for Rapid Determination of Bacterial Load. Frontiers in Microbiology, 10, 1-13. https://doi.org/10.3389/fmicb.2019.00801
Berney, M., Hammes, F., Bosshard, F., Weilenmann, H.-U. and Egli, T. (2007) Assessment and Interpretation of Bacterial Viability by Using The LIVE/DEAD BacLight Kit in Combination with Flow Cytometry. Applied and Environmental Microbiology, 73, 3283-3290. https://doi.org/10.1128/AEM.02750-06
Stiefel, P., Schmidt-Emrich, S., Maniura-Weber, K. and Ren, Q. (2015) Critical aspects of Using Bacterial Cell Viability Assays with the Fluorophores SYTO9 and Propidium Iodide. BMC Microbiol, 15, Article No. 36. https://doi.org/10.1186/s12866-015-0376-x
Netuschil, L., Auschill, T.M., Sculean, A. and Arweiler, N.B. (2014) Confusion over Live/Dead Stainings for the Detection of Vital Microorganisms in Oral Biofilms—Which Stain Is Suitable? BMC Oral Health, 14, Article No. 2. https://doi.org/10.1186/1472-6831-14-2
Sarker, A.S.D., Naharb, L. and Kumarasamy, Y. (2007) Microtitre Plate-Based Antibacterial Assay Incorporating Resazurin as an Indicator of Cell Growth, and Its Application in the in Vitro Antibacterial Screening of Phytochemicals. Methods, 42, 321-324. https://doi.org/10.1016/j.ymeth.2007.01.006
Lobato, R.C., Klafke, G.B. and Xavier, M.O. (2016) Reproducibility of Different Techniques Associated with Filtration in the Standardization of Aspergillus fumigatus Conidia Inoculum. Vittalle-Revista de Ciências da Saúde, 28, 84-89.
Marsh, P.D. and Martin, M.V. (2005) Oral Microbiology. 4th Edition, Livraria e Editora Santos, São Paulo, 192 p.
Reis, J. and Melo, P. (2003) Dental Caries, an Infectious Disease. Revista Portuguesa de Saúde Pública, 21, 84.
Canettieri, A.C.V., Kretchetoff, F.Y., Fujarra, F.C., Moreira, D. and Unterkircher, C.S. (2006) Effect of Mononucleal Antibodies 56G on the Growth of Streptococcus mutans in Broth and on Accumulation of Dental Plaque in Vitro. Ciência Odontológica Brasileira, 9, 67-75.
Koo, H., Gomes, B.P.F.A., Rosalen, P.L., Ambrosano, G.M.B., Park, Y.K. and Cury, J.A. (2000) In Vitro Antimicrobial Activity of Propolis and Arnica Montana against Oral Pathogens. Archives of Oral Biology, 45, 141-148. https://doi.org/10.1016/S0003-9969(99)00117-X
Pereira, J.V., Pereira, M.S.V., Sampaio, F.C., Sampaio, M.C.C., Alves, P.M., Araújo, C.R.F., et al. (2006) In vitro Antibacterial and Antiadherence Effect of the Extract of the Punica granatum Linn. upon Dental Biofilm Microrganisms. Revista Brasileira de Farmacognosia, 16, 88-93. https://doi.org/10.1590/S0102-695X2006000100016
Conde, N.C.O., Pereira, M.S.V., Bandeira. M.F.C.L., Venancio, G.N., Oliveira, G.P. and Sampaio, F.C. (2015) In Vitro Antimicrobial Activity of Plants of the Amazon on Oral Biofilm Micro-Organisms. Revista Odonto Ciencia, 30, 179-183.
Venancio, G.N., Rodrigues, I.C., Souza, T.P., Marreiro, R.O., Bandeira, M.F.C.L. and Conde, N.C.O. (2015) Herbal Mouthwash Based on Libidibia ferrea: Microbiological Control, Sensory Characteristics, Sedimentation, pH and Density. Revista de Odontologia da UNESP, 44, 118-124. https://doi.org/10.1590/1807-2577.1064