Antibiotic Resistance Phenotypes of <i>Enterobacteriaceae</i> Isolated from Household Wastewater in Brazzaville, Republic of Congo
- 1 Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, University Marien NGOUABI, Brazzaville, Congo
- 2 Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, University Marien NGOUABI, Brazzaville, Congo
- 3 Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, University Marien NGOUABI, Brazzaville, Congo
- 4 Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, University Marien NGOUABI, Brazzaville, Congo
Abstract
Household wastewater is a source of pollution and can present health risks when discharged into the environment. Thus, samples of household wastewater from a few neighborhoods in Brazzaville were analyzed for microbiological quality. The various samples were cultured for isolation on solid media using conventional microbiological methods. The bacteria isolated were identified by the Enterobacter System gallery. Sensitivity tests were performed using the standard antibiotic susceptibility test by diffusion on Mueller Hinton medium. At the end of the analysis, 51 Enterobacteriaceae were isolated and identified. They included: 8 (15.68%) Escherichia coli , 8 (15.68%) Salmonella spp. , 8 (15.68%) Shigella spp. , 8 (15.68%) Klebsiella spp. , 5 (9.80%) Enterobacter aerogenes , 8 (15.68%) Enterobacter cloacae , 3 (5.90%) Arizona spp. , 3 (5.90%) Proteus spp . The results obtained show that the bacteria tested showed total resistance to the following antibiotics: amoxicillin, amoxicillin + clavulanic acid, cloxacillin and nalidixic acid. On the other hand, imipenem, cefuroxime, cefotaxime, cefftriazone and kanamycin were the most active antibiotics with low levels of resistance. The low resistance rates observed for imipenem, cefotaxime, cefuroxime and cefftriazone show that these antibiotics can be used for the treatment of infections caused by household wastewater bacteria.
- Neuhauser, M.M., Weinstein, R.A., Rydman, R., Danziger, L.H., Karam, G. and Quinn, J.P. (2003) Antibiotic Resistance among Gram-Negative Bacilli in US Intensive Care Units: Implications for Fluoroquinolone Use. JAMA, 289, 885-888. https://doi.org/10.1001/jama.289.7.885
- Jansen, K.U., Knirsch, C. and Anderson, A.S. (2018) The Role of Vaccines in Preventing Bacterial Antimicrobial Resistance. Nature Medicine, 24, 10-19. https://doi.org/10.1038/nm.4465
- Johnson, J.R. and Russo, T.A. (2002) Uropathogenic Escherichia coli as Agents of Diverse Non-Urinary Tract Extraintestinal Infections. The Journal of Infectious Diseases, 186, 859-864. https://doi.org/10.1086/342490
- Ouali El Lalami, A., Zanibou, A., Bekhti, K., Zerrouq, F. and Marzouk, M. (2014) Microbiological Quality Control of Domestic and Industrial Wastewater from the City of FES in Morocco. Journal of Materials and Environmental Science, 5, 2325- 2332.
- Diallo, A.A. (2013) Pathogenic and Antibiotic-Resistant Escherichia coli in Effluents of Human and Animal Origin: Prevalence and Characterization before and after Purification Treatment. Single Thesis, University of Toulouse III, Toulouse, 204 p.
- Durmaz, B., Durmaz, R. and Sahin, K. (1997) Methicillin-Resistance among Turkish Isolates of Staphylococcus aureus Strains from Nosocomial and Community Infections and Their Resistance Patterns Using Various Antimicrobial Agents. Journal of Hospital Infection, 37, 325-329. https://doi.org/10.1016/S0195-6701(97)90149-3
- Prats, G., Mirelis, B., Llovet, T., Munoz, C., Miro, E. and Navarro, F. (2000) Antibiotic Resistance Trends in Enteropathogenic Bacteria Isolated in 1985-1987 and 1995-1998 in Barcelona. AntimicrobialAgents and Chemotherapy, 44, 1140-1145. https://doi.org/10.1128/AAC.44.5.1140-1145.2000
- Carret, G., Cavallo, J.D., Chardon, H., Chidiac, C., Choutet, P., Courvallin, P., Dabernat, H., Drugeon, H., Dubreuil, L., Golstein, F., Jarlier, V., Leclercq, R., Nchanoine, M.H., Philippon, A., Quentin, C., Rouveix, B., Sirot, J. and Soussy, C.J. (2001) Communiqué du comité de l’antibiogramme de la Société Française de Microbiologie. 47 p.
- Servais, P. and Passerat, J. (2009) Antimicrobial Resistance of Fecal Bacteria in Waters of the Seine River Basin (France). Science of the Total Environment, 408, 365-372.
- Tagajdid, M.R., Boumhil, L., Iken, M., Adnaoui, M. and Benoud, A. (2010) Study of the Resistance of Escherichia coli Strains Isolated in Urine to Third Generation Fluoroquinolones and Cephalosporins. Medicine and Infectious Diseases, 40, 70-73. https://doi.org/10.1016/j.medmal.2008.10.015