Enteric campylobacteriosis caused by Campylobacter is a real public health problem. It is a typical zoonosis that can be transmitted through direct contact with contaminated animals or animal carcasses, or indirectly through the ingestion of contaminated food or water. WHO 1 defines Campylobacter as the first germ responsible for gastroenteritis in developing countries. The objective of the study is to determine the prevalence of Campylobacter in pigs and to identify the most common species found in three slaughterhouses in the city of Yaounde. A descriptive and prospective study was carried out over a period of five months, from July to November 2020. To do this, Campylobacter spp. strains were searched for by culture on specific media, incubated in a micro-aerophilic atmosphere at 37°C and the identification with API campy (BioMérieux, ref 20800). Out of the 266 samples collected, 170 from the rectum and 96 from the carcasses, we obtained a general prevalence of 27%, with a prevalence of 25.88% in the rectum and 29.16% in the carcasses. Pigs intended for consumption by the population of Yaounde come mainly from the North (50%) and the West (49%). The most common species encountered were C. coli (52.77%), C. jejuni spp. jejuni 1 and 2 (30.58%), C. festus spp. festus (11.11%), C. jejuni spp. doley (1.38%), and Campylobacter spp. (4.16%). Bacterial enumeration gave Campylobacter fecal concentrations at 10 3 CFU 2 (52.7%), 10 4 CFU (33.3%), and 10 5 CFU (13.8%). The slaughterhouse in the Mvog Ada market was the most involved in Campybacter cases, followed by the 8 th market and Essos market. From the above, it is important to notice that the Campylobacter danger remains and that preventive measures are of great interest.
Organisation Mondiale de la Santé Animale (2013) le concept “Une sante” l’approche de l’OIE. Bulletin No. 2013-1.
Whiley, H., van den Akker, B., Giglio, S. and Bentham, R. (2013) The Role of Environmental Reservoirs in Human Campylobacteriosis. International Journal of Environmental Research and Public Health, 10, 5886-5907. https://doi.org/10.3390/ijerph10115886
Elbrissi, A., Sabeil, Y.A., Khalifa, K.A., Enan, K., Khair, O.M. and El Hussein, A.M. (2017) Isolation, Identification and Dierentiation of Campylobacter spp. Using Multiplex PCR Assay from Goats in Khartoum State, Sudan. Tropical Animal Health and Production, 49, 575-581. https://doi.org/10.1007/s11250-017-1231-x
Mossong, J., Mughini-Gras, L., Penny, C., Devaux, A., Olinger, C., Losch, S., Cauchie, H.-M., van Pelt, W. and Ragimbeau, C. (2016) Human Campylobacteriosis in Luxembourg, 2010-2013: A Case-Control Study Combined with Multilocus Sequence Typing for Source Attribution and Risk Factor Analysis. Scientific Reports, 6, Article No. 20939. https://doi.org/10.1038/srep20939
Sheppard, S.K., Dallas, J.F., Strachan, N.J.C., MacRae, M., McCarthy, N.D., Wilson, D.J., Gormley, F.J., Falush, D., Ogden, I.D., Maiden, M.C.J. and Forbes, K.J. (2009) Campylobacter Genotyping to Determine the Source of Human Infection. Clinical Infectious Diseases, 48, 1072-1078. https://doi.org/10.1086/597402
Nathues, C., Grüning, P., Fruth, A., Verspohl, J., Blaha, T., Kreienbrock, L. and Merle, R. (2013) Campylobacter spp., Yersinia enterocolitica, and Salmonella enterica and Their Simultaneous Occurrence in German Fattening Pig Herds and Their Environment. Journal of Food Protection, 76, 1704-1711. https://doi.org/10.4315/0362-028X.JFP-13-076
Carrique-Mas, J.J., Bryant, J.E., Cuong, N.V., Hoang, N.V.M., Campbell, J., Hoang, N.V., Dung, T.T.N., Duy, D.T., Hoa, N.T., Thompson, C., Hien, V.V., Phat, V.V., Farrar, J. and Baker, S. (2014) An Epidemiological Investigation of Campylobacter in Pig and Poultry Farms in the Mekong Delta of Vietnam. Epidemiology & Infection, 142, 1425-1436. https://doi.org/10.1017/S0950268813002410
Milnes, A.S., Stewart, I., Clifton-Hadley, F.A., Davies, R.H., Newell, D.G., Sayers, A.R., Cheasty, T., Cassar, C., Ridley, A., Cook, A.J.C., Evans, S.J., Teale, C.J., Smith, R.P., McNally, A., Toszeghy, M., Futter, R., Kay, A. and Paiba, G.A. (2008) Intestinal Carriage of Verocytotoxigenic Escherichia coli O157, Salmonella, Thermophilic Campylobacter and Yersinia enterocolitica, in Cattle, Sheep and Pigs at Slaughter in Great Britain during 2003. Epidemiology & Infection, 136, 739-751. https://doi.org/10.1017/S0950268807009223
Abley, M.J., Wittum, T.E., Funk, J.A. and Gebreyes, W.A. (2012) Antimicrobial Susceptibility, Pulsed-Field Gel Electrophoresis, and Multi-Locus Sequence Typing of Campylobacter coli in Swine before, during, and after the Slaughter Process. Foodborne Pathogens and Disease, 9, 506-512. https://doi.org/10.1089/fpd.2011.1053
Quintana-Hayashi, M.P. and Thakur, S. (2012) Longitudinal Study of the Persistence of Antimicrobial-Resistant Campylobacter Strains in Distinct Swine Production Systems on Farms, at Slaughter, and in the Environment. Applied and Environmental Microbiology, 78, 2698-2705. https://doi.org/10.1128/AEM.07723-11
EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control) (2015) The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-Borne Outbreaks in 2014. EFSA Journal, 13, Article No. 4329. https://doi.org/10.2903/j.efsa.2015.4329
Bolton, F.J., Coated, D., Hinchliffe, P.M. and Robertson, L. (1983) Comparison of Selective Media for Isolation of Campylobacter jejuni/coli. Journal of Clinical Pathology, 36, 78-83. https://doi.org/10.1136/jcp.36.1.78
Joséphine, E.A.M., Uwizeyimana, L. and Sans, P. (2006) “ESSOR’’ “Espaces, Sociétés rurales et Logiques économiques’’ filière porcine camerounaise: Une compétitivité à l’épreuve de dysfonctionnements. Mémoire de master 2 recherche en géographie et aménagement.
Andrzejewska, M., Szczepańska, B., Spica, D. and Klawe, J.J. (2019) Prevalence, Virulence, and Antimicrobial Resistance of Campylobacter spp. in Raw Milk, Beef, and Pork Meat in Northern Poland. Foods, 8, Article No. 420. https://doi.org/10.3390/foods8090420
Karikari, A.B., Obiri-Danso, K., Frimpong, E.H. and Krogfelt, K.A. (2017) Antibiotic Resistance of Campylobacter Recovered from Faeces and Carcasses of Healthy Livestock. BioMed Research International, 2017, Article ID: 4091856. https://doi.org/10.1155/2017/4091856
Koffi, K.E., Coulibaly, K.J., Saraka, N.D., Senin, C.B.V., Boka, H. and Dosso, M. (2020) évaluation de la qualité microbiologique des carcasses de porcs abattus à la SIVAC: recherche de Salmonella, Yersinia et Campylobacter. Afrique Science, 16, 30-38. http://www.afriquescience.net
Denis, M., Chidaine, B., Laisney, M.-J., Kempf, I., Rivoal, K., Mégraud, F. and Fravalo, P. (2009) Comparison of Genetic Profiles of Campylobacter Strains Isolated from Poultry, Pig and Campylobacter Human Infections in Brittany, France. Pathologie Biologie, 57, 23-29. https://doi.org/10.1016/j.patbio.2008.04.007
Tadesse, D.A., Wittum, T., Bahnson, P.B., DeGraves, F., Funk, J.A., Thakur, S., Rajala-Schultz, P., Morgan Morrow, W.E. and Gebreyes, W.A. (2011) Prevalence and Antimicrobial Resistance Profile of Campylobacter spp. Isolated from Conventional and Antimicrobial-Free Swine Production Systems from Different U.S. Regions. Foodborne Pathogens and Disease, 8, 367-374. https://doi.org/10.1089/fpd.2010.0665
Burrough, E., Terhorst, S., Sahin, O. and Zhang, Q. (2013) Prevalence of Campylobacter spp. Relative to Other Enteric Pathogens in Grow-Finish Pigs with Diarrhea. Anaerobe, 22, 111-114. https://doi.org/10.1016/j.anaerobe.2013.06.004
Thakur, S. and Gebreyes, W.A. (2005) Prevalence and Antimicrobial Resistance of Campylobacter in Antimicrobial-Free and Conventional Pig Production Systems. Journal of Food Protection, 68, 2402-2410. https://doi.org/10.4315/0362-028X-68.11.2402
Alter, T., Gaull, F., Kasimir, S., Gurtler, M., Mielke, H., Linnebur, M., et al. (2005) Prevalences and Transmission Routs of Campylobacter spp. Strains within Multiple Pig Farms. Veterinary Microbiology, 108, 251-261. https://doi.org/10.1016/j.vetmic.2005.03.004
Jensen, A.N., Dalsgaard, A., Baggesen, D.L. and Nielsen, E.M. (2006) The Occurrence and Characterization of Campylobacter jejuni and C. coli in Organic Pigs and Their Outdoor Environment. Veterinary Microbiology, 116, 96-105. https://doi.org/10.1016/j.vetmic.2006.03.006
Niesen, E.M., Engberg, J. and Madsen, M. (1997) Distribution of Serotypes of Campylobacter jejuni and C. coli from Danish Patients, Poultry, Cattle and Swine. FEMS Immunology & Medical Microbiology, 19, 47-56. https://doi.org/10.1111/j.1574-695X.1997.tb01071.x