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Anomeric Proton and Carbon (H1-C1) NMR Chemical Shifts of Antigenic Mannans Obtained from Pathogenic Yeast <i>Candida tropicalis</i>
Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
Divisions of Infection Control and Prevention, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
- 1 Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
- 2 Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
- 3 Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
- 4 Divisions of Infection Control and Prevention, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
- 5 Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan
Advances in Microbiology·Volume 11 (2021)·Pages 296–301·Published 11 May 2021·DOI10.4236/aim.2021.115022
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Abstract
On two dimensional maps of 1 H- 13 C correlation spectroscopy (H-C COSY) analysis for the mannan of Candida tropicalis , nine cross peaks of anomeric proton and carbon were useful for the purpose of obtaining information on the chemical structure of this molecule. Namely, the mannans was comb-like structure constructed with the linear α -1,6-linked polymannnosyl backbone and several oligomannnosyl side chains composed of α -1,2-, α -1,3-, and β -1,2-linkages. Therefore, in the structural investigation of comb-like mannan, two-dimensional H-C COSY analysis is as useful as two-dimensional nuclear Hartmann-Hahn (HOHAHA) analysis.
Keywords<i>Candida tropicalis</i>Cell Wall MannanComb-Like Structure<sup>1</sup>H-<sup>13</sup>C Correlation SpectroscopyAnomeric Carbon Chemical Shift<i>α</i>
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