Phenotypic and Genotypic Characterization of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase among Enterobacteria Isolated at National Public Health Laboratory of Brazzaville — Oak Academic Publishing
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Phenotypic and Genotypic Characterization of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase among Enterobacteria Isolated at National Public Health Laboratory of Brazzaville
Service de Bactériologie, Laboratoire National de Santé Publique, Brazzaville, Congo
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Laboratoire de Microbiologie, Infectiologie et Immunologie, Ecole Normale Supérieure, Université Marien Ngouabi, Brazzaville, Congo
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Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
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Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
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Laboratoire de Microbiologie, Infectiologie et Immunologie, Ecole Normale Supérieure, Université Marien Ngouabi, Brazzaville, Congo
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Service de Bactériologie, Laboratoire National de Santé Publique, Brazzaville, Congo
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Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
1 Service de Bactériologie, Laboratoire National de Santé Publique, Brazzaville, Congo
2 Laboratoire de Microbiologie, Infectiologie et Immunologie, Ecole Normale Supérieure, Université Marien Ngouabi, Brazzaville, Congo
3 Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
4 Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
5 Laboratoire de Microbiologie, Infectiologie et Immunologie, Ecole Normale Supérieure, Université Marien Ngouabi, Brazzaville, Congo
6 Service de Bactériologie, Laboratoire National de Santé Publique, Brazzaville, Congo
7 Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo
The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This work aimed to assess prevalence of beta-lactamase produced by enterobacterial isolates. Then, disc diffusion, double disc synergy test (DDST) and combined disc test (CDT) were respectively used for antimicrobial resistance, detection of Extended-Spectrum Beta-Lactamases (ESBL) and Metallo-Beta-Lactamases (MBL). bla genes were detected by PCR. A total of 132 enterobacterial strains were studied. Resistance to antibiotic families was observed with a greater frequency than 50%. Gentamicin was the least active beta-lactam antibiotic, with a resistance rate of 88%. 40.9% of strains show an ESBL phenotype and 16.6% were MBL. An overall prevalence of 74% (40/54) and respectively rates of 29.6%, 27.7% and 16.7% for bla SHV, bla CTX and bla TEM genes were observed. SHV, CTX, CTX/SHV/TEM, CTX/TEM, SHV/TEM and CTX/SHV were different ESBL genotypes observed. ESBL-producing enterobacteria isolation worried about the future of antimicrobial therapy in the Republic of Congo. This is a public health problem that requires careful monitoring and implementation of a policy of rational antibiotics use.
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